Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
College of Food Science and Engineering, Shandong Agricultural University, Taian, Shandong 271018, China.
J Agric Food Chem. 2024 Jun 5;72(22):12842-12858. doi: 10.1021/acs.jafc.4c00079. Epub 2024 May 20.
Granule-associated surface lipids (GASLs) and internal lipids showed different lipid-amylose relationships, contents, and distributions, suggesting their differing biological origins and functions, among waxy, normal, and high-amylose rice starch. The GASL content mainly depended on the pore size, while internal lipids regulated starch biosynthesis, as indicated by correlations of internal lipids with the chain length distribution of amylopectin and amylose content. Of the 1346 lipids detected, 628, 562, and 408 differentially expressed lipids were observed between normal-waxy, high-amylose-waxy, and normal-high-amylose starch, respectively. After the removal of GASLs, the higher lysophospholipid content induced greater decreases in the peak and breakdown viscosity and swelling power, while the highest digestibility increase was found with the highest triacylglycerol content. Thus, different GASL compositions led to different digestibility, swelling, and pasting outcomes. This study sheds new light on the mechanism of the role of GASLs in the structure and properties of starch, as well as in potential modifications and amyloplast membrane development.
颗粒相关表面脂质 (GASL) 和内部脂质表现出不同的脂质-直链淀粉关系、含量和分布，这表明它们在蜡质、正常和高直链淀粉稻米淀粉中具有不同的生物起源和功能。GASL 含量主要取决于孔径，而内部脂质则调节淀粉生物合成，这可以通过内部脂质与支链淀粉的链长分布和直链淀粉含量之间的相关性来证明。在所检测到的 1346 种脂质中，分别在正常-蜡质、高直链淀粉-蜡质和正常-高直链淀粉淀粉之间观察到 628、562 和 408 种差异表达的脂质。在去除 GASLs 后，较高的溶血磷脂含量导致峰值和崩解粘度以及膨胀力更大的降低，而最高的消化率增加则出现在最高的三酰甘油含量时。因此，不同的 GASL 组成导致不同的消化率、膨胀和糊化特性。本研究为 GASLs 在淀粉结构和性质以及潜在的改性和质体膜发育中的作用机制提供了新的见解。
