From the Department of Plastic, Reconstructive and Aesthetic Surgery, Basaksehir Cam and Sakura City Hospital, Istanbul, Turkey.
Private Practice, Antalya, Turkey.
Ann Plast Surg. 2024 Jun 1;92(6):700-702. doi: 10.1097/SAP.0000000000003898.
There is currently no standardization in the field of research on fat grafts in rats, which is one of the most popular topics in plastic surgery. The aim of our study was to demonstrate the effects of selecting paraepididymal fat grafts as the donor area on enhancing the reliability of fat graft studies.
In this study, 12 male Sprague-Dawley rats were used to obtain adipose grafts from both inguinal and paraepididymal regions. After measuring the graft weights, they were subjected to histological examination using hematoxylin-eosin staining, as well as immunohistochemical staining with antiperilipin antibody. Purity of the samples, viability of adipose cells, and the presence of lymph nodes within the grafts were analyzed.
The purity of adipose cells in graft samples obtained from the paraepididymal region was found to be 98.1% compared with the total sample. In contrast, the purity of adipose cells in graft samples obtained from the inguinal region was 58.37%. Hematoxylin-eosin staining revealed significantly higher adipocyte viability and vascularity in the paraepididymal region compared with the inguinal region (P = 0.0134). Conversely, lymphatic tissue content in samples obtained from the inguinal region was significantly higher compared with paraepididymal adipose tissue samples (P < 0.0001). Immunohistochemical staining with antiperilipin antibody showed a denser and more uniform staining pattern in paraepididymal adipose grafts (P < 0.0001).
Using paraepididymal fat, devoid of lymphatic tissue, naturally eliminates 2 critical biases (estrogen and lymphatic tissue), enhancing the standardization and reliability of fat graft survival studies.
在大鼠脂肪移植研究领域目前还没有标准化，这是整形外科学中最热门的课题之一。我们的研究旨在展示选择附睾旁脂肪移植作为供区对提高脂肪移植研究可靠性的作用。
本研究使用 12 只雄性 Sprague-Dawley 大鼠，分别从腹股沟区和附睾旁区获取脂肪移植物。测量移植物重量后，对其进行苏木精-伊红染色的组织学检查，以及抗 perilipin 抗体的免疫组织化学染色。分析样本的纯度、脂肪细胞的活力以及移植物内是否存在淋巴结。
与总样本相比，来自附睾旁区的脂肪细胞移植物样本的纯度为 98.1%，而来自腹股沟区的脂肪细胞移植物样本的纯度为 58.37%。苏木精-伊红染色显示附睾旁区的脂肪细胞活力和血管化明显高于腹股沟区（P = 0.0134）。相反，腹股沟区脂肪组织样本中的淋巴组织含量明显高于附睾旁脂肪组织样本（P < 0.0001）。抗 perilipin 抗体的免疫组织化学染色显示附睾旁脂肪移植物的染色模式更加致密和均匀（P < 0.0001）。
使用不含淋巴组织的附睾旁脂肪可自然消除 2 个关键偏倚（雌激素和淋巴组织），从而提高脂肪移植成活率研究的标准化和可靠性。
