State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
Food Funct. 2024 Jun 17;15(12):6347-6358. doi: 10.1039/d4fo00673a.
Age-related macular degeneration (AMD) is a prominent cause of vision loss among the elderly, and the treatment options for dry AMD (dAMD) are severely limited. Lutein has a favorable effect on the treatment of dAMD. Algae oil, rich in docosahexaenoic acid (DHA), is considered an effective intervention for eye diseases. In this study, casein-mannose conjugates were prepared to form algal oil-in-water Pickering emulsions by ultrasound-assisted Maillard reaction. As the ultrasound time increased from 0 to 25 min, the droplet size decreased to 648.2 ± 21.18 nm, which substantially improved the stability of the Pickering emulsions. The retention of lutein in the Pickering emulsions under ultrasonic treatment for 20 min was significantly improved under different conditions. The simulated gastrointestinal digestion revealed that ultrasound-assisted Pickering emulsions are an effective method for improving the bioaccessibility of lutein (19.76%-53.34%). studies elucidated that the lutein-loaded Pickering emulsions could effectively alleviate retinal thinning induced by sodium iodate (NaIO) in mice with dAMD. Mechanistically, lutein-loaded Pickering emulsions significantly reduced oxidative stress by decreasing the MDA level, increasing the SOD production, and reducing the retinal ROS production. These findings explored the protective effects of lutein-loaded Pickering emulsions on dAMD and offered promising prospects for the nutritional intervention of dAMD.
年龄相关性黄斑变性（AMD）是老年人视力丧失的主要原因，而干性 AMD（dAMD）的治疗选择非常有限。叶黄素对治疗 dAMD 有良好的效果。富含二十二碳六烯酸（DHA）的藻油被认为是治疗眼部疾病的有效干预措施。在这项研究中，通过超声辅助美拉德反应制备了酪蛋白甘露糖缀合物，以形成藻油/Water Pickering 乳液。随着超声时间从 0 增加到 25 分钟，液滴尺寸减小到 648.2±21.18nm，这极大地提高了 Pickering 乳液的稳定性。在不同条件下，超声处理 20 分钟后，叶黄素在 Pickering 乳液中的保留率显著提高。模拟胃肠道消化表明，超声辅助 Pickering 乳液是提高叶黄素生物利用度（19.76%-53.34%）的有效方法。研究表明，载叶黄素的 Pickering 乳液可以有效缓解 dAMD 小鼠因碘酸钠（NaIO）引起的视网膜变薄。机制上，载叶黄素的 Pickering 乳液通过降低 MDA 水平、增加 SOD 产生和减少视网膜 ROS 产生来显著减轻氧化应激。这些发现探讨了载叶黄素的 Pickering 乳液对 dAMD 的保护作用，并为 dAMD 的营养干预提供了有前景的前景。
