Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, China.
Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian 116023, China.
Comp Biochem Physiol B Biochem Mol Biol. 2024 Aug-Sep;273:110988. doi: 10.1016/j.cbpb.2024.110988. Epub 2024 May 18.
The Pacific oyster Crassostrea gigas is renowned for its high zinc content, but the significant variation among individuals diminishes its value as a reliable source of zinc supplementation. The Zrt/Irt-like protein 1 (ZIP1), a pivotal zinc transporter that facilitates zinc uptake in various organisms, plays crucial roles in regulating zinc content. In the present study, polymorphisms of a ZIP1 gene in C. gigas (CgZIP1-II) were investigated, and their association with zinc content was evaluated through preliminary association analysis in 41 oysters and verification analysis in another 200 oysters. A total of 17 single nucleotide polymorphisms (SNPs) were identified in the exonic region of CgZIP1-II gene, with c.503A>G significantly associated with zinc content. Protein sequence and structure prediction showed that c.503A>G caused a p.Met110Val nonsynonymous mutation located in the metal-binding region of CgZIP1-II, which could influence its affinity for zinc ions, thereby modulating its zinc transport functionality. These results indicate the potential influence of CgZIP1-II polymorphisms on zinc content and provide candidate markers for selecting C. gigas with high zinc content.
太平洋牡蛎（Crassostrea gigas）以其高锌含量而闻名，但个体间的显著差异降低了其作为可靠锌补充来源的价值。Zrt/Irt 样蛋白 1（ZIP1）是一种重要的锌转运蛋白，可促进各种生物的锌摄取，在调节锌含量方面发挥着关键作用。本研究调查了太平洋牡蛎（Crassostrea gigas）中 ZIP1 基因的多态性，并通过对 41 只牡蛎进行初步关联分析和对另外 200 只牡蛎进行验证分析，评估了其与锌含量的关联。在 CgZIP1-II 基因的外显子区域共鉴定出 17 个单核苷酸多态性（SNP），c.503A>G 与锌含量显著相关。蛋白质序列和结构预测表明，c.503A>G 导致位于 CgZIP1-II 金属结合区域的 p.Met110Val 非同义突变，可能影响其对锌离子的亲和力，从而调节其锌转运功能。这些结果表明 CgZIP1-II 多态性对锌含量的潜在影响，并为选择高锌含量的太平洋牡蛎提供了候选标记。
