通过大规模固定化pH梯度提高PI（α1-抗胰蛋白酶）表型的分辨率。
Improved resolution of PI (alpha 1-antitrypsin) phenotypes by a large-scale immobilized pH gradient.
作者信息
Görg A, Postel W, Weser J, Patutschnick W, Cleve H
出版信息
Am J Hum Genet. 1985 Sep;37(5):922-30.
Abstract
PI variants and PI M subtypes were examined by isoelectric focusing in a preformed immobilized pH gradient. Conditions were found that permit reliable classification of PI variants and of most PI M subtypes: the pH gradient ranges from 4.3 to 4.8 on a polyacrylamide gel with a length of 20 cm and a separation distance of approximately 16-17 cm (delta pH = 0.025 U/cm). The PI phenotypes observed are presented.
摘要
通过在预制的固定化pH梯度中进行等电聚焦来检测PI变体和PI M亚型。发现了能够对PI变体和大多数PI M亚型进行可靠分类的条件：在长度为20 cm、分离距离约为16 - 17 cm（ΔpH = 0.025 U/cm）的聚丙烯酰胺凝胶上，pH梯度范围为4.3至4.8。展示了观察到的PI表型。
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