Foliar Inoculation of into Corn Induces Infection and Local Spread in Field Environments.

Tar spot of corn ( L.) is a significant disease in the United States and Canada caused by , an obligate biotroph fungus. However, field research critical for understanding and managing the disease has been hindered by a need for methods to inoculate corn with in field environments. In this study, we developed and demonstrated the efficacy of a method to initiate tar spot in field settings using inoculations of corn leaves with inoculum that had been stored at -20°C for 10 months. Stromata of were observed 19 days after inoculations in two field experiments, and stromata resulting from secondary spread were initially observed 39 to 41 days after the initial inoculations. Tar spot was not present in the fields beyond the inoculated areas or localized spread area, signifying that the establishment of initial disease resulted solely from inoculations. This study enhances our understanding of inoculation and infection of corn with and tar spot development in field environments. The results will aid new research into understanding the corn tar spot pathosystem and improving management strategies.