大鼠促肾上腺皮质激素释放因子前体cDNA的克隆与序列分析
Cloning and sequence analysis of cDNA for rat corticotropin-releasing factor precursor.
作者信息
Jingami H, Mizuno N, Takahashi H, Shibahara S, Furutani Y, Imura H, Numa S
出版信息
FEBS Lett. 1985 Oct 21;191(1):63-6. doi: 10.1016/0014-5793(85)80994-7.
DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including a putative signal peptide. The CRF and putative signal peptide regions are more highly conserved among rat, human and ovine prepro-CRF than is the cryptic portion.
通过用人基因组DNA探针筛选cDNA文库，已克隆出与编码促肾上腺皮质激素释放因子前体（前促肾上腺皮质激素释放因子）的大鼠下丘脑mRNA互补的DNA。对克隆的cDNA进行核苷酸序列分析表明，大鼠前促肾上腺皮质激素释放因子由187个氨基酸残基组成，包括一个假定的信号肽。促肾上腺皮质激素释放因子和假定的信号肽区域在大鼠、人类和绵羊前促肾上腺皮质激素释放因子中比隐蔽部分更为保守。
Zotero 插件