高压氧通过减轻轻度认知障碍（aMCI）大鼠的神经元凋亡改善长期学习和记忆障碍。

Hyperbaric Oxygen Improves Long-Term Learning and Memory Impairment by Attenuating Neuronal Apoptosis in aMCI Rats.

作者信息

Zhang Yonggui, Lin Xianzhong, Ye Chen, Zhang Peiling

机构信息

Department of Anesthesiology, The Second Hospital of Longyan, Fujian, People's Republic of China.

Department of Anesthesiology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, People's Republic of China.

出版信息

J Inflamm Res. 2024 May 15;17:3043-3055. doi: 10.2147/JIR.S455155. eCollection 2024.

DOI:10.2147/JIR.S455155

PMID:38770175

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11103017/

Abstract

BACKGROUND

With the aging of the population and the increasing incidence of neurological diseases, amnestic mild cognitive impairment (aMCI) has attracted attention. Hyperbaric oxygen (HBO) has gradually shown the potential in the treatment of aMCI as an emerging treatment method in recent times. This study is to observe the effect of HBO on the long-term learning memory of aMCI rats, and investigate the associated mechanisms.

METHODS

Seventy-two male rats (4-month-old) were randomly divided into control (CON) group, aMCI group, HBO group, 24 rats in each group. Each group was randomly divided into CON, CON, CON; aMCI, aMCI, aMCI; HBO, HBO, HBO, 8 rats in each group. The aMCI model rats were established in aMCI and HBO groups. HBO group was treated with HBO for 7 days. The ethological and cytopathology which include Morris water maze (MWM) test, HE staining, TUNEL staining and the expression of Fas/FasL on neuron membrane were conducted to evaluate the effects of HBO on day 1, day 7 and day 28 after HBO treatment.

RESULTS

MWM test showed that the spatial learning and memory ability of the rats decreased in aMCI group, and recovered in HBO group; Compared with aMCI group, the pathological damage of hippocampal nerve cells was alleviated, the number of apoptotic cells was significantly reduced (P < 0.05), and the expression of Fas/FasL on the surface of nerve cell membrane was significantly weakened in HBO group (P < 0.05). There were no significant changes in the spatial learning and memory ability, pathological damage of hippocampal neurons, the number of apoptotic cells, and the changes of Fas/FasL on the surface of hippocampal neurons in HBO, HBO, and HBO groups (P > 0.05). However, in aMCI aMCI, and aMCI groups gradually aggravated (P < 0.05).

CONCLUSION

HBO can improve the long-term learning and memory impairment by attenuating neuronal apoptosis in aMCI rats. 2. Fas/FasL mediated cell receptor death pathway is involved in the apoptosis of hippocampal neurons.

摘要

背景

随着人口老龄化和神经疾病发病率的增加，遗忘型轻度认知障碍（aMCI）受到关注。近年来，高压氧（HBO）作为一种新兴的治疗方法，在aMCI治疗中逐渐显示出潜力。本研究旨在观察HBO对aMCI大鼠长期学习记忆的影响，并探讨其相关机制。

方法

将72只4月龄雄性大鼠随机分为对照组（CON）、aMCI组、HBO组，每组24只。每组再随机分为CON、CON、CON；aMCI、aMCI、aMCI；HBO、HBO、HBO，每组8只。在aMCI组和HBO组建立aMCI模型大鼠。HBO组给予HBO治疗7天。在HBO治疗后第1天、第7天和第28天，进行行为学和细胞病理学检测，包括Morris水迷宫（MWM）试验、HE染色、TUNEL染色以及神经元膜上Fas/FasL的表达，以评估HBO的作用效果。

结果

MWM试验显示，aMCI组大鼠的空间学习和记忆能力下降，而HBO组恢复；与aMCI组相比，HBO组海马神经细胞的病理损伤减轻，凋亡细胞数量显著减少（P<0.05），神经细胞膜表面Fas/FasL的表达明显减弱（P<0.05）。HBO、HBO、HBO组大鼠的空间学习和记忆能力、海马神经元的病理损伤、凋亡细胞数量以及海马神经元表面Fas/FasL的变化均无显著差异（P>0.05）。然而，aMCI、aMCI、aMCI组的上述指标逐渐加重（P<0.05）。