Department of Ophthalmology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Department of Ophthalmology, University of Iowa Hospitals and Clinics, Iowa City, Iowa, USA.
Indian J Ophthalmol. 2024 Nov 1;72(11):1624-1629. doi: 10.4103/IJO.IJO_1092_23. Epub 2024 May 20.
To study the morphological and histological characteristics of the fibrous capsule formed around a novel biodegradable glaucoma drainage device (GDD) implant made of oxidized regenerated cellulose (ORC) after 1 and 3 months of its implantation.
It was a prospective, interventional, preclinical experimental study using New Zealand white rabbits. All animals underwent GDD implantation with a novel biodegradable device. The device's body was made of ORC and attached to a silicone tube, connecting the body to the anterior chamber. Histopathology (hematoxylin and eosin/Masson's trichrome staining) and immunohistochemistry (Alpha-Smooth Muscle Actin expression) characteristics of the bleb formed around the novel device were noted at 1 and 3 months.
One month post implantation, the biodegradable material produced a significant foreign body type of reaction evidenced by the exuberant infiltration by macrophages, lymphocytes, and multinucleated giant cells. The granulomatous response subsided by 3 months with disorganized collagen deposition on Masson's trichrome staining. The silicone tube was an internal control, and histopathology demonstrated well-organized collagen deposition around it at 3 months. Immunohistochemistry for α-smooth muscle actin also demonstrated more myofibroblast transformation at the site of the tube than the biodegradable implant.
Our results indicate that the tissue response around a biodegradable GDD was different from the response to conventional devices. A diffuse, loose vascularized mesh was observed to develop, which may be more compatible with sustained IOP control over a longer period, in contrast to the usual thick and dense capsule formed around the non-biodegradable devices.
研究新型可生物降解氧化再生纤维素（ORC）青光眼引流装置（GDD）植入 1 个月和 3 个月后纤维囊的形态和组织学特征。
这是一项前瞻性、干预性、临床前实验研究，使用新西兰白兔。所有动物均接受新型可生物降解装置的 GDD 植入。该装置的主体由 ORC 制成，并附有一个硅胶管，将主体与前房连接。在 1 个月和 3 个月时观察新型装置周围形成的囊泡的组织病理学（苏木精和伊红/Masson 三色染色）和免疫组织化学（α-平滑肌肌动蛋白表达）特征。
植入后 1 个月，可生物降解材料产生了明显的异物反应，表现为巨噬细胞、淋巴细胞和多核巨细胞的过度浸润。3 个月时，肉芽肿反应消退，Masson 三色染色显示胶原排列紊乱。硅胶管是内部对照，3 个月时其周围的组织病理学显示胶原排列整齐。α-平滑肌肌动蛋白免疫组化也表明，与可生物降解植入物相比，管内的肌成纤维细胞转化更多。
我们的结果表明，可生物降解 GDD 周围的组织反应与传统装置不同。观察到一种弥漫、疏松的血管化网形成，这可能与较长时间内更持续的眼压控制更相容，而不是通常在不可生物降解装置周围形成的厚而致密的囊。
