Mindfulness is a popular technique that helps people to get closer to their self. However, recent findings indicate that mindfulness may not benefit everybody. In the present research, we hypothesized that mindfulness promotes alienation from the self among individuals with low abilities to self-regulate affect (state-oriented individuals) but not among individuals with high abilities to self-regulate affect (action-oriented individuals). In two studies with participants who were mostly naïve to mindfulness practices (70% indicated no experience; N1 = 126, 42 men, 84 women, 0 diverse, aged 17-86 years, Mage = 31.87; N2 = 108, 30 men, 75 women, 3 diverse, aged 17-69 years, Mage = 28.00), we tested a mindfulness group (five-minute mindfulness exercise) against a control group (five-minute text reading). We operationalized alienation as lower consistency in repeated preference judgments and a lower tendency to adopt intrinsic over extrinsic goal recommendations. Results showed that, among state-oriented participants, mindfulness led to significantly lower consistency of preference judgments (Study 1) and lower adoption of intrinsic over extrinsic goals (Study 2) compared to text reading. The alienating effect was absent among action-oriented participants. Thus, mindfulness practice may alienate psychologically vulnerable people from their self and hamper access to preferences and intrinsic goals. We discuss our findings within Personality-Systems-Interactions (PSI) theory.