Anhui Innovative Center for Drug Basic Research of Metabolic Diseases, Wannan Medical College, Wuhu 241002, China; School of Pharmacy, Wannan Medical College, Wuhu 241002, China.
School of Anesthesiology, Wannan Medical College, Wuhu 241002, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Oct 5;318:124479. doi: 10.1016/j.saa.2024.124479. Epub 2024 May 19.
Nowadays, it has been proven that lipid droplets (LDs) not only maintain the fundamental cellular functions, but also play an essential role in the pathogenesis of numerous diseases. Non-alcoholic fatty liver disease (NAFLD) is among these diseases. In this work, we designed two polarity sensitive fluorescent probes TST and TSO with D-π-A-D structure by introducing different electron acceptor groups according to the low polarity of LDs. The experimental discovered that probe TST exhibited the characteristics of near-infrared emission, high selectivity towards polarity, large Stokes shift, rapid targeting ability of LDs, and robust wash-free biological imaging capability. Confocal images illustrated that probe TST has been successfully applied in monitoring LDs polarity during ferroptosis, as well as visualizing changes in LDs polarity at both tissue and organ levels in fatty liver conditions. With these exceptional properties, probe TST was anticipated to make further contributions to the field of LDs research.
如今，已经证明脂滴（LDs）不仅维持基本的细胞功能，而且在许多疾病的发病机制中起着至关重要的作用。非酒精性脂肪性肝病（NAFLD）就是其中之一。在这项工作中，我们根据 LDs 的低极性，通过引入不同的电子受体基团，设计了具有 D-π-A-D 结构的两种极性敏感荧光探针 TST 和 TSO。实验发现，探针 TST 表现出近红外发射、对极性的高选择性、大斯托克斯位移、快速靶向 LDs 的能力以及强大的无洗生物成像能力。共聚焦图像表明，探针 TST 已成功应用于监测铁死亡过程中 LDs 的极性，以及可视化脂肪肝条件下组织和器官水平上 LDs 极性的变化。探针 TST 具有这些优异的性能，有望为 LDs 研究领域做出进一步的贡献。
