School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China; Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Keling Road No. 88, Suzhou 215163, China.
Jiangsu Key Lab of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Keling Road No. 88, Suzhou 215163, China.
Int Immunopharmacol. 2024 Jun 30;135:112283. doi: 10.1016/j.intimp.2024.112283. Epub 2024 May 20.
Toll-like receptors (TLRs) play a crucial role in mediating immune responses by recognizing pathogen-associated molecular patterns (PAMPs) and danger-associated molecular patterns (DAMPs), as well as facilitating apoptotic cell (ACs) clearance (efferocytosis), thus contributing significantly to maintaining homeostasis and promoting tissue resolution. In this study, we investigate the impact of TLR agonists on macrophage efferocytosis. Our findings demonstrate that pretreatment with the TLR agonist lipopolysaccharide (LPS) significantly enhances macrophage phagocytic ability, thereby promoting efferocytosis both in vitro and in vivo. Moreover, LPS pretreatment confers tissue protection against damage by augmenting macrophage efferocytic capacity in murine models. Further examination reveals that LPS modulates efferocytosis by upregulating the expression of Tim4.These results underscore the pivotal role of TLR agonists in regulating the efferocytosis process and suggest potential therapeutic avenues for addressing inflammatory diseases. Overall, our study highlights the intricate interplay between LPS pretreatment and efferocytosis in maintaining tissue homeostasis and resolving inflammation.
Toll 样受体 (TLRs) 通过识别病原体相关分子模式 (PAMPs) 和危险相关分子模式 (DAMPs) ，以及促进凋亡细胞 (ACs) 清除 (吞噬作用) ，在介导免疫反应中发挥着至关重要的作用，从而对维持内稳态和促进组织修复有重要贡献。在这项研究中，我们研究了 TLR 激动剂对巨噬细胞吞噬作用的影响。我们的研究结果表明，TLR 激动剂脂多糖 (LPS) 的预处理可显著增强巨噬细胞的吞噬能力，从而促进体外和体内的吞噬作用。此外，LPS 预处理通过增强巨噬细胞的吞噬能力来保护组织免受损伤，在小鼠模型中发挥作用。进一步的研究表明，LPS 通过上调 Tim4 的表达来调节吞噬作用。这些结果强调了 TLR 激动剂在调节吞噬作用过程中的关键作用，并为解决炎症性疾病提供了潜在的治疗途径。总的来说，我们的研究强调了 LPS 预处理和吞噬作用在维持组织内稳态和解决炎症中的复杂相互作用。
