Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, College of Agriculture, Yangzhou University, Yangzhou 225009, China; Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China.
Department of Food Science and Engineering, Yangzhou University, Yangzhou 225009, China.
Int J Biol Macromol. 2024 Jun;270(Pt 2):132445. doi: 10.1016/j.ijbiomac.2024.132445. Epub 2024 May 20.
Glycogen is a highly branched glucose polymer that is an energy storage material in fungi and animals. Extraction of glycogen from its source in a way that minimizes its molecular degradation is essential to investigate its native structure. In this study, the following extraction methods were compared: sucrose gradient density ultracentrifugation, thermal alkali, hot alcohol and hot water extractions. Molecular-size and chain-length distributions of glycogen were measured by size-exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis, respectively. These two fine-structure features are the most likely structural characteristics to be degraded during extraction. The results show that the thermal alkali, hot alcohol and hot water extractions degrade glycogen molecular size and/or chain-length distributions, and that sucrose gradient density ultracentrifugation with neither high temperature nor alkaline treatment is the most suitable method for fungal glycogen extraction.
糖原是一种高度分支的葡萄糖聚合物，是真菌和动物的能量储存物质。从其来源中提取糖原时，最大限度地减少其分子降解对于研究其天然结构至关重要。在这项研究中，比较了以下提取方法：蔗糖梯度密度超速离心、热碱、热酒精和热水提取。通过尺寸排阻色谱法和荧光辅助碳水化合物电泳法分别测量糖原的分子大小和链长分布。这两个精细结构特征是在提取过程中最有可能降解的结构特征。结果表明，热碱、热酒精和热水提取会降解糖原的分子大小和/或链长分布，而既不高温也不碱处理的蔗糖梯度密度超速离心是真菌糖原提取最适宜的方法。
