Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, China.
Plant Conservation Technology Center, Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang, 550006, China.
Sci Rep. 2024 May 21;14(1):11587. doi: 10.1038/s41598-024-62512-2.
Peptide deformylase can catalyse the removal of formyl groups from the N-terminal formyl methionine of the primary polypeptide chain. The peptide deformylase genes of a few herbaceous plants have been studied to some extent, but the peptide deformylase genes of woody plants have not been studied. In this study, we isolated EuPDF1B from Eucommia ulmoides Oliv. The full-length sequence of EuPDF1B is 1176 bp long with a poly-A tail and contains an open reading frame of 831 bp that encodes a protein of 276 amino acids. EuPDF1B was localized to the chloroplast. qRT‒PCR analysis revealed that this gene was expressed in almost all tissues tested but mainly in mature leaves. Moreover, the expression of EuPDF1B was enhanced by ABA, MeJA and GA and inhibited by shading treatment. The expression pattern of EuPDF1B was further confirmed in EuPDF1Bp: GUS transgenic tobacco plants. Among all the transgenic tobacco plants, EuPDF1Bp-3 showed the highest GUS histochemical staining and activity in different tissues. This difference may be related to the presence of enhancer elements in the region from - 891 bp to - 236 bp of the EuPDF1B promoter. In addition, the expression of the chloroplast gene psbA and the net photosynthetic rate, fresh weight and height of tobacco plants overexpressing EuPDF1B were greater than those of the wild-type tobacco plants, suggesting that EuPDF1B may promote the growth of transgenic tobacco plants. This is the first time that PDF and its promoter have been cloned from woody plants, laying a foundation for further analysis of the function of PDF and the regulation of its expression.
肽脱甲酰基酶可以催化从一级多肽链的 N 端甲酰甲硫氨酸上去除甲酰基。已经对一些草本植物的肽脱甲酰基酶基因进行了一定程度的研究，但木本植物的肽脱甲酰基酶基因尚未得到研究。在本研究中，我们从杜仲中分离出 EuPDF1B。EuPDF1B 的全长序列长 1176bp，带有 poly-A 尾巴，包含一个 831bp 的开放阅读框，编码 276 个氨基酸的蛋白质。EuPDF1B 定位于叶绿体。qRT-PCR 分析表明，该基因在几乎所有测试的组织中都有表达，但主要在成熟叶片中表达。此外，ABA、MeJA 和 GA 增强了 EuPDF1B 的表达，而遮光处理则抑制了其表达。EuPDF1B 在 EuPDF1Bp:GUS 转基因烟草植物中的表达模式进一步得到了证实。在所有转基因烟草植物中，EuPDF1Bp-3 在不同组织中表现出最高的 GUS 组织化学染色和活性。这种差异可能与 EuPDF1B 启动子从-891bp 到-236bp 区域存在增强子元件有关。此外，过表达 EuPDF1B 的烟草植物的叶绿体基因 psbA 和净光合速率、鲜重和高度的表达均大于野生型烟草植物，表明 EuPDF1B 可能促进转基因烟草植物的生长。这是首次从木本植物中克隆 PDF 及其启动子，为进一步分析 PDF 的功能及其表达调控奠定了基础。
