Zhang Dingyu, Zhu Ziwei, Yang Bing, Li Xiaofeng, Zhang Hongmei, Zhu Hongfang
Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai, 201403, China.
State Key Laboratory of Genetic Engineering and Fudan Center for Genetic Diversity and Designing Agriculture, School of Life Sciences, Fudan University, Shanghai, 200438, China.
Mol Hortic. 2024 May 22;4(1):21. doi: 10.1186/s43897-024-00092-5.
Salicylic acid (SA) is a multi-functional phytohormone, regulating diverse processes of plant growth and development, especially triggering plant immune responses and initiating leaf senescence. However, the early SA signaling events remain elusive in most plant species apart from Arabidopsis, and even less is known about the multi-facet mechanism underlying SA-regulated processes. Here, we report the identification of a novel regulatory module in cucumber, CsNPR1-CsWRKY11, which mediates the regulation of SA-promoted leaf senescence and ROS burst. Our analyses demonstrate that under SA treatment, CsNPR1 recruits CsWRKY11 to bind to the promoter of CsWRKY11 to activate its expression, thus amplifying the primary SA signal. Then, CsWRKY11 cooperates with CsNPR1 to directly regulate the expression of both chlorophyll degradation and ROS biosynthesis related genes, thereby inducing leaf de-greening and ROS burst. Our study provides a solid line of evidence that CsNPR1 and CsWRKY11 constitute a key module in SA signaling pathway in cucumber, and gains an insight into the interconnected regulation of SA-triggered processes.
水杨酸（SA）是一种多功能植物激素，可调节植物生长发育的多种过程，尤其是触发植物免疫反应和启动叶片衰老。然而，除拟南芥外，大多数植物物种中早期SA信号事件仍不清楚，关于SA调控过程的多方面机制了解更少。在这里，我们报告了在黄瓜中鉴定出一个新的调控模块CsNPR1-CsWRKY11，它介导SA促进的叶片衰老和活性氧爆发的调控。我们的分析表明，在SA处理下，CsNPR1招募CsWRKY11结合到CsWRKY11的启动子上以激活其表达，从而放大初级SA信号。然后，CsWRKY11与CsNPR1合作直接调节叶绿素降解和活性氧生物合成相关基因的表达，从而诱导叶片褪绿和活性氧爆发。我们的研究提供了确凿的证据，证明CsNPR1和CsWRKY11构成黄瓜SA信号通路中的一个关键模块，并深入了解了SA触发过程的相互关联调控。
