代谢性酸中毒时肾脏甘氨酸裂解酶复合物活性增加。
Increased activity of renal glycine-cleavage-enzyme complex in metabolic acidosis.
作者信息
Lowry M, Hall D E, Brosnan J T
出版信息
Biochem J. 1985 Oct 15;231(2):477-80. doi: 10.1042/bj2310477.
Abstract
Glycine is metabolized in isolated renal cortical tubules to stochiometric qualities of ammonia, CO2 and serine by the combined actions of the glycine-cleavage-enzyme complex and serine hydroxymethyltransferase. The rate of renal glycine metabolism by this route is increased in tubules from acidotic rats, but is not affected in vitro by decreasing the incubation pH from 7.4 to 7.1. Metabolic acidosis caused an increase in the renal activity of the glycine-cleavage-enzyme complex, but there were no changes in the activity of serine hydroxymethyltransferase or of methylenetetrahydrofolate dehydrogenase. This enzymic adaptation permits increased ammoniagenesis from glycine during acidosis. The physiological implications are discussed.
摘要
在离体肾皮质肾小管中，甘氨酸通过甘氨酸裂解酶复合体和丝氨酸羟甲基转移酶的联合作用，代谢生成化学计量的氨、二氧化碳和丝氨酸。酸中毒大鼠肾小管中通过该途径进行的肾甘氨酸代谢速率增加，但在体外将孵育pH从7.4降至7.1时，该代谢速率不受影响。代谢性酸中毒导致肾甘氨酸裂解酶复合体的活性增加，但丝氨酸羟甲基转移酶或亚甲基四氢叶酸脱氢酶的活性没有变化。这种酶适应性使得酸中毒期间从甘氨酸生成氨的过程增加。文中讨论了其生理意义。
相似文献
1
Increased activity of renal glycine-cleavage-enzyme complex in metabolic acidosis.代谢性酸中毒时肾脏甘氨酸裂解酶复合物活性增加。
Biochem J. 1985 Oct 15;231(2):477-80. doi: 10.1042/bj2310477.
2
Role of the glycine-cleavage system in glycine and serine metabolism in various organs.甘氨酸裂解系统在各器官甘氨酸和丝氨酸代谢中的作用。
Biochem Soc Trans. 1980 Oct;8(5):504-6. doi: 10.1042/bst0080504.
3
Mechanism of the glycine cleavage reaction: retention of C-2 hydrogens of glycine on the intermediate attached to H-protein and evidence for the inability of serine hydroxymethyltransferase to catalyze the glycine decarboxylation.甘氨酸裂解反应的机制：甘氨酸C-2位氢原子在与H蛋白相连的中间体上的保留以及丝氨酸羟甲基转移酶无法催化甘氨酸脱羧反应的证据。
Arch Biochem Biophys. 1986 Nov 15;251(1):121-7. doi: 10.1016/0003-9861(86)90058-5.
4
Resolution and characterization of the glycine-cleavage reaction in pea leaf mitochondria. Properties of the forward reaction catalysed by glycine decarboxylase and serine hydroxymethyltransferase.豌豆叶片线粒体中甘氨酸裂解反应的解析与表征。甘氨酸脱羧酶和丝氨酸羟甲基转移酶催化的正向反应特性。
Biochem J. 1988 Oct 1;255(1):169-78. doi: 10.1042/bj2550169.
5
Nonketotic hyperglycinemia: two patients with primary defects of P-protein and T-protein, respectively, in the glycine cleavage system.非酮症高甘氨酸血症：分别有两名患者在甘氨酸裂解系统中存在P蛋白和T蛋白的原发性缺陷。
Pediatr Res. 1983 Dec;17(12):967-70. doi: 10.1203/00006450-198312000-00008.
6
7
Regulation of the balance of one-carbon metabolism in Saccharomyces cerevisiae.酿酒酵母中一碳代谢平衡的调控
J Biol Chem. 2000 Oct 6;275(40):30987-95. doi: 10.1074/jbc.M004248200.
8
Light-induced increases in the glycine decarboxylase multienzyme complex from pea leaf mitochondria.光诱导豌豆叶片线粒体中甘氨酸脱羧酶多酶复合体增加。
Arch Biochem Biophys. 1986 Aug 1;248(2):626-38. doi: 10.1016/0003-9861(86)90517-5.
9
A strategy for glycine encephalopathy therapy.甘氨酸脑病治疗策略。
J Ment Defic Res. 1982 Jun;26 (Pt 2):107-10. doi: 10.1111/j.1365-2788.1982.tb00134.x.
10
Effects of the metabolites of the branched-chain amino acids and cysteamine on the glycine cleavage system.支链氨基酸和半胱胺的代谢产物对甘氨酸裂解系统的影响。
Biochem Int. 1983 Feb;6(2):225-30.
引用本文的文献
1
Synthesis of glycine from 4-hydroxyproline in tissues of neonatal pigs with intrauterine growth restriction.用宫内发育受限的新生仔猪组织内的 4-羟脯氨酸合成甘氨酸。
Exp Biol Med (Maywood). 2023 Sep;248(17):1446-1458. doi: 10.1177/15353702231199080. Epub 2023 Oct 14.
2
Synthesis of glycine from 4-hydroxyproline in tissues of neonatal pigs.从新生仔猪组织中的 4-羟脯氨酸合成甘氨酸。
Exp Biol Med (Maywood). 2023 Jul;248(14):1206-1220. doi: 10.1177/15353702231181360. Epub 2023 Aug 25.
本文引用的文献
1
N15 TRACER STUDIES ON THE ORIGIN OF URINARY AMMONIA IN THE ACIDOTIC DOG, WITH NOTES ON THE ENZYMATIC SYNTHESIS OF LABELED CLUTAMIC ACID AND GLUTAMINES.关于酸中毒犬尿氨来源的N15示踪研究，附标记谷氨酸和谷氨酰胺酶促合成的注释
J Clin Invest. 1965 May;44(5):731-45. doi: 10.1172/JCI105186.
2
3
Amino acid extraction and ammonia metabolism by the human kidney during the prolonged administration of ammonium chloride.氯化铵长期给药期间人肾脏的氨基酸提取和氨代谢
J Clin Invest. 1963 Feb;42(2):263-76. doi: 10.1172/JCI104713.
4
The mechanism of ammonia excretion during ammonium chloride acidosis.氯化铵酸中毒时氨的排泄机制。
J Clin Invest. 1955 Jan;34(1):20-6. doi: 10.1172/JCI103058.
5
Studies in biochemical adaptation; the origin or urinary ammonia as indicated by the effect of chronic acidosis and alkalosis on some renal enzymes in the rat.生化适应性研究；慢性酸中毒和碱中毒对大鼠某些肾酶的影响所显示的尿氨的来源。
Biochem J. 1952 Nov;52(3):407-12. doi: 10.1042/bj0520407.
6
Activation of oxoglutarate dehydrogenase in the kidney in response to acute acidosis.急性酸中毒时肾脏中α-酮戊二酸脱氢酶的激活。
Biochem J. 1980 Sep 15;190(3):771-80. doi: 10.1042/bj1900771.
7
ROSFIT: an enzyme kinetics nonlinear regression curve fitting package for a microcomputer.ROSFIT：一种用于微型计算机的酶动力学非线性回归曲线拟合程序包。
Comput Biomed Res. 1982 Feb;15(1):39-45. doi: 10.1016/0010-4809(82)90051-9.
8
Glycine metabolism in rat kidney cortex slices.大鼠肾皮质切片中的甘氨酸代谢
Biochem J. 1982 Apr 15;204(1):313-21. doi: 10.1042/bj2040313.
9
Liver enzymes of serine metabolism during neonatal development of the rat.大鼠新生儿发育过程中丝氨酸代谢的肝脏酶
Biochem J. 1980 Aug 15;190(2):451-5. doi: 10.1042/bj1900451.
10
Regulation of the glycine cleavage system in isolated rat liver mitochondria.大鼠离体肝线粒体中甘氨酸裂解系统的调节
J Biol Chem. 1983 Mar 10;258(5):2993-9.
Zotero 插件