大鼠肾皮质切片中的甘氨酸代谢
Glycine metabolism in rat kidney cortex slices.
作者信息
Rowsell E V, Al-Naama M M, Rowsell K V
出版信息
Biochem J. 1982 Apr 15;204(1):313-21. doi: 10.1042/bj2040313.
When rat kidney cortex slices were incubated with glycine or [1-14C]glycine, after correcting for metabolite changes with control slices, product formation and glycine utilization fitted the requirements of the equation: 2 Glycine leads to ammonia + CO2 + serine. Evidence is presented that degradation via glyoxylate, by oxidation or transamination, is unlikely to have any significant role in kidney glycine catabolism. It is concluded that glycine metabolism in rat kidney is largely via glycine cleavage closely coupled with serine formation. 1-C decarboxylation and urea formation with glycine in rat hepatocyte suspensions were somewhat greater than decarboxylation or ammonia formation in kidney slices, showing that in the rat, potentially, the liver is quantitatively the more important organ in glycine catabolism. There was no evidence of ammonia formation from glycine with rat brain cortex, heart, spleen or diaphragm and 1-C decarboxylation was very weak.
当用甘氨酸或[1-¹⁴C]甘氨酸孵育大鼠肾皮质切片时，在用对照切片校正代谢物变化后，产物形成和甘氨酸利用符合以下方程式的要求：2甘氨酸→氨 + 二氧化碳 + 丝氨酸。有证据表明，通过乙醛酸氧化或转氨作用进行的降解在肾脏甘氨酸分解代谢中不太可能起任何重要作用。得出的结论是，大鼠肾脏中的甘氨酸代谢主要通过与丝氨酸形成紧密偶联的甘氨酸裂解进行。大鼠肝细胞悬液中甘氨酸的¹⁴C脱羧作用和尿素形成比肾切片中的脱羧作用或氨形成略大，表明在大鼠中，肝脏在数量上可能是甘氨酸分解代谢中更重要的器官。没有证据表明大鼠脑皮质、心脏、脾脏或膈肌能从甘氨酸形成氨，且¹⁴C脱羧作用非常微弱。
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