Bahler D W, Lord E M
Cancer Res. 1985 Dec;45(12 Pt 1):6362-5.
Dimethyl sulfoxide (DMSO) has previously been shown to increase the surface expression of H-2K and H-2D antigens on cultured line 1 carcinoma cells. H-2 densities increase from initial levels barely detectable with flow cytometry to those found on normal BALB/c spleen cells. Here we compare the susceptibilities of untreated and DMSO-treated line 1 cells to lysis mediated by H-2d specific monoclonal antibodies and complement, cytotoxic T-cells, and natural killer cells. Induced H-2 antigens appear to function normally in that DMSO-treated cells are highly susceptible to all types of H-2 restricted immune lysis, whereas untreated line 1 cells are not. DMSO does not increase lysis of line 1 cells mediated by natural killer cells. Our results suggest that DMSO could be used to make the growth of major histocompatibility complex antigen-deficient tumors more sensitive to T-cell-mediated immunological resistance.
二甲基亚砜（DMSO）先前已被证明可增加培养的1号线癌细胞上H-2K和H-2D抗原的表面表达。H-2密度从最初用流式细胞术几乎检测不到的水平增加到正常BALB/c脾细胞上发现的水平。在这里，我们比较了未处理和DMSO处理的1号线细胞对由H-2d特异性单克隆抗体和补体、细胞毒性T细胞和自然杀伤细胞介导的裂解的敏感性。诱导的H-2抗原似乎功能正常，因为DMSO处理的细胞对所有类型的H-2限制性免疫裂解高度敏感，而未处理的1号线细胞则不然。DMSO不会增加自然杀伤细胞介导的1号线细胞的裂解。我们的结果表明，DMSO可用于使主要组织相容性复合体抗原缺陷肿瘤的生长对T细胞介导的免疫抗性更敏感。
