Department of Biology, Faculty of Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran.
Cell Tissue Bank. 2024 Sep;25(3):765-772. doi: 10.1007/s10561-024-10130-7. Epub 2024 May 22.
Tissue engineering is a set of techniques for producing or reconstructing tissue that primarily aims to restore or improve the function of tissues in the human body. The aim of the present study was to evaluate the mechanical and histological characteristics of decellularized tracheal scaffolds prepared in comparison with fresh trachea for use in tracheal repair. In order to prepare the scaffold, sheep's trachea was prepared and after cleaning the waste tissues, they were decellularized. Then decellularized scaffolds were evaluated histologically and laboratory and numerical study of the nonlinear mechanical behavior of tracheal tissue and scaffold and their comparison. Examining the results of histological evaluations showed that the decellularization of the scaffolds was completely done. These results were confirmed by hematoxylin-eosin staining. Also, the exact hyperelastic properties of tracheal tissue and scaffold were used in biomechanical models, and according to the presented results, the five-term Mooney-Rivlin strain energy density function became a suitable behavioral model for modeling the hyperelastic behavior of trachea and scaffold. In total, the results of this research showed that the scaffolds obtained from decellularization by preserving the main compositions of the desired tissue can be a suitable platform for investigating cell behaviors.
组织工程是一组用于生产或重建组织的技术，主要旨在恢复或改善人体组织的功能。本研究的目的是评估与新鲜气管相比，脱细胞气管支架的机械和组织学特性，以用于气管修复。为了制备支架，准备了绵羊的气管，并在清除了废物组织后，对其进行了脱细胞处理。然后对脱细胞支架进行组织学评估，并对气管组织和支架的非线性力学行为及其比较进行实验室和数值研究。对组织学评估结果的检查表明，支架的脱细胞处理已完全完成。这些结果通过苏木精-伊红染色得到了证实。此外，气管组织和支架的精确超弹性特性被用于生物力学模型中，根据提出的结果，五参数 Mooney-Rivlin 应变能密度函数成为了用于模拟气管和支架超弹性行为的合适行为模型。总的来说，这项研究的结果表明，通过保留所需组织的主要成分进行脱细胞处理获得的支架可以成为研究细胞行为的合适平台。
