Collier G E, Nishimura J S
J Biol Chem. 1979 Nov 10;254(21):10925-30.
Ethoxyformic anhydride was used to demonstrate the existence of a second important histidine in succinyl-CoA synthetase from Escherichia coli. Differential labeling of the enzyme by [3H]ethoxyformic anhydride gave a stoichiometry of one important histidine per alpha beta catalytic unit. Data are presented suggesting that this residue and an important thiol group on the beta subunit (Collier, G., and Nishimura, J.S. (1978) J. Biol. Chem. 253, 4938-4943) interact with each other during catalysis. A mechanism of action involving these 2 residues is proposed for one of the partial reactions catalyzed by succinyl-CoA synthetase.
乙氧基甲酸酐被用于证明来自大肠杆菌的琥珀酰辅酶A合成酶中第二个重要组氨酸的存在。用[³H]乙氧基甲酸酐对该酶进行差异标记，得到的化学计量比为每个αβ催化单元一个重要组氨酸。所呈现的数据表明，在催化过程中，这个残基与β亚基上的一个重要巯基（科利尔，G.，和西村，J.S.（1978年）《生物化学杂志》253，4938 - 4943）相互作用。针对琥珀酰辅酶A合成酶催化的其中一个部分反应，提出了一种涉及这两个残基的作用机制。
