College of Life Sciences, Tarim University, Alar, Xinjiang, China.
Department of Veterinary Medical Science, Shandong Vocational Animal Science and Veterinary College, Weifang, Shandong, China.
PLoS One. 2024 May 22;19(5):e0303567. doi: 10.1371/journal.pone.0303567. eCollection 2024.
Spermatozoa cryopreservation has been practiced for decades and is a very useful technique for long-term preservation of sperm fertility. The capability for semen cryopreservation varies across species, seasons, latitudes, and even for different ejaculates from the same animal. This article summarizes research results on sperm cryotolerance biomarkers in several species, focusing on three areas: spermatozoa cryotolerance biomarkers, seminal plasma proteins cryotolerance biomarkers, and other cryotolerance biomarkers. We discovered that sperm cryoresistance biomarkers are primarily related to sperm plasma membrane stability, the presence of antioxidant substances in sperm or seminal plasma, sperm cell energy metabolism, water and small molecule transport channels in the sperm plasma membrane, and antistress substances in sperm or seminal plasma. The research conducted using diverse livestock models can be employed to enhance the basic and applied reproduction of other mammals through the study of sperm cryotolerance biomarkers, as well as the substantial similarities between livestock and other organisms, including endangered species.
精子冷冻保存已经有几十年的历史了，是一种非常有用的技术，可以长期保存精子的生育能力。不同物种、不同季节、不同纬度，甚至同一动物的不同精液，其精子冷冻保存能力都有所不同。本文总结了几种物种的精子抗冻性生物标志物的研究结果，重点关注三个领域：精子抗冻性生物标志物、精浆蛋白抗冻性生物标志物和其他抗冻性生物标志物。我们发现，精子抗冻性生物标志物主要与精子质膜稳定性、精子或精浆中抗氧化物质的存在、精子细胞能量代谢、精子质膜中水和小分子运输通道以及精子或精浆中的抗应激物质有关。利用不同的家畜模型进行的研究可以通过研究精子抗冻性生物标志物来增强其他哺乳动物的基础和应用繁殖，并且家畜和其他生物，包括濒危物种，之间存在着很大的相似性。
