Genetics and Embryo Technologies Application and Research Center, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.
Department of Reproduction and Artificial Insemination, University of Ankara, Ankara, Turkey.
Andrologia. 2021 Oct;53(9):e14164. doi: 10.1111/and.14164. Epub 2021 Jul 2.
In this study, the quality of frozen bull semen was evaluated with the proAKAP4 level test. Sixty straws of frozen bull semen from various batches (n = 30) belonging to six bulls were used in the current study. The frozen bull semen samples were analysed in terms of proAKAP4 levels, sperm morphology and sperm movement parameters at hour 0 and hour 3 after thawing. The semen samples were divided into three groups according to the proAKAP4 levels: low concentration (<25 ng/10x10 spermatozoa), moderate concentration (25 to 39 ng/10x10 spermatozoa) and high concentration (≥40 ng/10x10 spermatozoa). A positive correlation was found between the proAKAP4 level and total motility (TM ), progressive motility (PM ), VSL and VCL values obtained after the third-hour thermoresistance test (p < .05). There was a negative correlation between the percentage of sperm abnormal tail and the proAKAP4 level (p < .01). In addition, it was observed that the semen samples with proAKAP4 concentrations of 25 ng/10 spermatozoa and higher preserved the TM and PM motility characteristics. In conclusion, the proAKAP4 has the potential to become a biomarker protein to evaluate in the quality analysis of frozen-thawed semen.
本研究采用 proAKAP4 水平检测评估冷冻公牛精液的质量。本研究使用了来自 6 头公牛的 60 支不同批次（n=30）的冷冻公牛精液 straw。在解冻后 0 小时和 3 小时，对冷冻公牛精液样本进行 proAKAP4 水平、精子形态和精子运动参数分析。根据 proAKAP4 水平将精液样本分为三组：低浓度（<25ng/10x10 精子）、中浓度（25-39ng/10x10 精子）和高浓度（≥40ng/10x10 精子）。在第三个小时的耐热试验后，proAKAP4 水平与总活力（TM）、前向运动（PM）、VSL 和 VCL 值呈正相关（p<.05）。精子尾部异常百分比与 proAKAP4 水平呈负相关（p<.01）。此外，观察到 proAKAP4 浓度为 25ng/10 精子的精液样本保持了 TM 和 PM 运动特征。总之，proAKAP4 有可能成为评估冷冻精液质量的生物标志物蛋白。
