Kimura M, Gleichmann E
J Immunol. 1985 Dec;135(6):3845-9.
We asked the question whether or not the Lyb-3+5+ B cell subset, which is lacking in CBA/N immune defective mice, is required for the lupus-like autoantibody formation caused by graft-vs-host reaction (GVHR). (CBA/N X DBA/2)F1 male defective mice injected with DBA/2 T cells produced IgG autoantibodies to the same extent as did nondefective F1 mice suffering from GVHR. Although a very small number of DBA/2 B cells might have contaminated the T cell inocula, it was shown that these were B cells of the defective F1 mice that produced autoantibodies during the GVHR. This was demonstrated by detecting autoantibodies carrying an immunoglobulin allotype of the F1 recipient. Furthermore, the defective F1 male mice injected with CBA/N lymphoid cells, which were lacking Lyb-3+5+ B cells, also produced autoantibodies. Isotype analysis of antinuclear antibodies revealed that some of them belonged to IgG3 isotype. It was concluded that the ontogenically late-appearing B cell subset is not required for GVH autoimmunity.
我们提出了一个问题，即CBA/N免疫缺陷小鼠中缺乏的Lyb-3+5+B细胞亚群对于移植物抗宿主反应（GVHR）引起的狼疮样自身抗体形成是否是必需的。注射了DBA/2 T细胞的（CBA/N×DBA/2）F1雄性缺陷小鼠产生IgG自身抗体的程度与患有GVHR的非缺陷F1小鼠相同。尽管极少量的DBA/2 B细胞可能污染了T细胞接种物，但结果表明，这些是在GVHR期间产生自身抗体的缺陷F1小鼠的B细胞。通过检测携带F1受体免疫球蛋白同种异型的自身抗体证明了这一点。此外，注射缺乏Lyb-3+5+B细胞的CBA/N淋巴细胞的缺陷F1雄性小鼠也产生了自身抗体。抗核抗体的同种型分析表明，其中一些属于IgG3同种型。得出的结论是，个体发育后期出现的B细胞亚群对于GVH自身免疫不是必需的。
