Sawada Y, Föhring B, Shenk T E, Raska K
Virology. 1985 Dec;147(2):413-21. doi: 10.1016/0042-6822(85)90143-6.
Sensitivity of a library of rat cells transformed in vitro with viable recombinant adenoviruses to natural killer (NK) cells and allogeneic cytotoxic T cells has been studied and correlated with their oncogenic potential in syngeneic rats. All cell lines transformed with the sub370-12E1AB virus (containing E1A and E1B regions of Ad12) and with the sub370-12E1A virions (containing the E1A region of Ad12 and the E1B region of Ad5) showed a high degree of resistance to NK cells. The cell lines transformed with the sub370-12E1B virus (containing the E1A region of Ad5 and the E1B region of Ad12) were highly sensitive to NK cytotoxicity. While all cell lines transformed with virions containing the E1A genes of Ad5 expressed high levels of class I MHC antigen, only three of eight cell lines containing the E1A region of Ad12 showed detectable levels by flow cytometric analysis after staining with specific antibodies. All cell lines containing E1A genes of Ad5 were killed by in vitro generated allogeneic cytolytic T cells. Only three of eight cell lines containing the E1A region of Ad12 were killed by such CTLs; the level of cytotoxicity, however, did not reach that seen with the cells containing the E1A genes of Ad5. All cell lines containing the E1A and E1B genes of Ad12 were highly tumorigenic. Only two of four cell lines transformed with virus containing the E1A genes of Ad12 and E1B region of Ad5 were tumorigenic. The efficiency of tumor induction was low and the latent period was long confirming the importance of the E1B region. None of the cell lines transformed with virus containing the E1A region of Ad5 and the E1B genes of Ad12 were tumorigenic, reflecting their high degree of sensitivity to both natural and induced cellular immunity. Expression of the E1A region of Ad12 in transformed cells modulates not only the level of class I MHC antigens, but also confers resistance to NK cell cytotoxicity.
对用活的重组腺病毒体外转化的大鼠细胞文库对自然杀伤（NK）细胞和同种异体细胞毒性T细胞的敏感性进行了研究，并将其与它们在同基因大鼠中的致癌潜力相关联。所有用sub370 - 12E1AB病毒（包含Ad12的E1A和E1B区域）和sub370 - 12E1A病毒粒子（包含Ad12的E1A区域和Ad5的E1B区域）转化的细胞系对NK细胞表现出高度抗性。用sub370 - 12E1B病毒（包含Ad5的E1A区域和Ad12的E1B区域）转化的细胞系对NK细胞毒性高度敏感。虽然所有用包含Ad5的E1A基因的病毒粒子转化的细胞系都高水平表达I类MHC抗原，但在用特异性抗体染色后，通过流式细胞术分析，八个包含Ad12的E1A区域的细胞系中只有三个显示出可检测水平。所有包含Ad5的E1A基因的细胞系都被体外产生的同种异体溶细胞性T细胞杀死。八个包含Ad12的E1A区域的细胞系中只有三个被这种CTL杀死；然而，细胞毒性水平未达到包含Ad5的E1A基因的细胞系所观察到的水平。所有包含Ad12的E1A和E1B基因的细胞系都具有高度致瘤性。用包含Ad12的E1A基因和Ad5的E1B区域的病毒转化的四个细胞系中只有两个具有致瘤性。肿瘤诱导效率低且潜伏期长，证实了E1B区域的重要性。用包含Ad5的E1A区域和Ad12的E1B基因的病毒转化的细胞系均无致瘤性，这反映了它们对天然和诱导的细胞免疫的高度敏感性。Ad12的E1A区域在转化细胞中的表达不仅调节I类MHC抗原的水平，还赋予对NK细胞毒性的抗性。
