Division of Animal Diseases & Health, National Institute of Animal Science, Rural Development Administration, Wanju, Republic of Korea.
Department of Veterinary Infectious Disease, College of Veterinary Medicine, Jeonbuk National University, Iksan, Republic of Korea.
Mycoses. 2024 May;67(5):e13748. doi: 10.1111/myc.13748.
Dermatophytosis is a common and major public health concern worldwide. Despite the increasing availability of antifungal drugs, relapses and untreated cases of dermatophyte infections are reported. Therefore, novel antifungal agents are required. Aminopyrrolnitrin (APRN) shows promise for dermatophytosis treatment because of its antifungal activity.
This study aimed to assess the antifungal properties of APRN against Trichophyton verrucosum (T. verrucosum), in both laboratory settings and a guinea pig model.
The minimum inhibitory concentrations (MICs) of APRN and enilconazole against T. verrucosum were determined according to the CLSI M38 method. The skins of 16 male guinea pigs were infected with 1.0 × 10 conidia of T. verrucosum and the animals were grouped into sets of four: negative control group (NC) received normal saline; positive control group (PC) received 2 μg/mL of enilconazole; and APRN4 and APRN8 received 4 and 8 μg/mL of APRN, respectively. Clinical, mycological and histological efficacies were measured after 10 days.
The MIC of APRN and enilconazole against T. verrucosum was 4 and 2 μg/mL, respectively. The clinical scores of PC, APRN4, and APRN8 were significantly lower than those of NC. Clinical and mycological efficacies were higher for APRN8, APRN4 and PC. No fungi were observed in the skin tissues of APRN4 and APRN8, while fungi were observed in 50% of the PC.
APRN showed antifungal activity against T. verrucosum in vitro and in vivo and is a potential candidate for the treatment of dermatophytosis.
皮肤癣菌病是全球常见且重大的公共卫生问题。尽管抗真菌药物的可及性不断增加，但仍有皮肤癣菌感染的复发和未治疗病例报告。因此，需要新型抗真菌药物。氨基蝶啶（APRN）因其抗真菌活性，有望用于治疗皮肤癣菌病。
本研究旨在评估 APRN 对疣状毛癣菌（T. verrucosum）的抗真菌特性，包括在实验室和豚鼠模型中。
根据 CLSI M38 方法，测定 APRN 和依利康唑对 T. verrucosum 的最低抑菌浓度（MIC）。将 16 只雄性豚鼠的皮肤感染 1.0×10 个 T. verrucosum 孢子，并将动物分为四组：阴性对照组（NC）给予生理盐水；阳性对照组（PC）给予 2μg/mL 的依利康唑；APRN4 和 APRN8 分别给予 4 和 8μg/mL 的 APRN。在第 10 天测量临床、真菌学和组织学疗效。
APRN 和依利康唑对 T. verrucosum 的 MIC 分别为 4 和 2μg/mL。PC、APRN4 和 APRN8 的临床评分显著低于 NC。APRN8、APRN4 和 PC 的临床和真菌学疗效更高。APRN4 和 APRN8 组的皮肤组织中未观察到真菌，而 PC 组有 50%观察到真菌。
APRN 在体外和体内均显示出对 T. verrucosum 的抗真菌活性，是治疗皮肤癣菌病的潜在候选药物。
