Shen Jing, Li Weijie, Tan Jia, Xia Chunyu, Wang Jianxin, Zhou Xinli
Institute of Biothermal Science and Technology, University of Shanghai for Science and Technology, Shanghai 200093, China.
Shanghai Technical Service Platform for Cryopreservation of Biological Resources, Shanghai 200093, China.
Sheng Wu Gong Cheng Xue Bao. 2024 May 25;40(5):1469-1485. doi: 10.13345/j.cjb.230657.
Ovarian tissue cryopreservation (OTC) is currently the exclusive choice for preserving fertility in both young girls before reaching puberty and young women who require immediate chemotherapy. Ovarian tissue transplantation has proven to be effective in restoring hormonal cycles and fertility. However, in certain cancer cases, there is a potential risk of inadvertently reintroducing malignant cells when transplanting cryopreserved ovarian tissue. Therefore, the use of an artificial ovary as an innovative and complementary approach allows for the development of isolated follicles, facilitates oocyte maturation and ovulation, and can partially restore endocrine function. This paper presents a comprehensive overview of techniques used to preserve fertility in natural ovarian tissues, including slow freezing, vitrification and hydrogel encapsulation methods. Additionally, it reviews fertility preservation techniques for artificial ovarian tissues, such as strategies involving hydrogel-encapsulated follicle, scaffolding for constructing ovarian microtissues, and 3D printing engineering. Lastly, this article explores current challenges and difficulties encountered in preserving ovarian tissue fertility, while also anticipating future trends in development, making it a valuable reference for the implementation of ovarian tissue fertility preservation.
卵巢组织冷冻保存(OTC)目前是青春期前年轻女孩以及需要立即进行化疗的年轻女性保留生育能力的唯一选择。卵巢组织移植已被证明在恢复激素周期和生育能力方面是有效的。然而,在某些癌症病例中,移植冷冻保存的卵巢组织时存在无意中重新引入恶性细胞的潜在风险。因此,使用人工卵巢作为一种创新的补充方法,可以促进孤立卵泡的发育,促进卵母细胞成熟和排卵,并能部分恢复内分泌功能。本文全面概述了用于保存天然卵巢组织生育能力的技术,包括慢速冷冻、玻璃化和水凝胶包封方法。此外,还综述了人工卵巢组织的生育力保存技术,如涉及水凝胶包封卵泡的策略、构建卵巢微组织的支架以及3D打印工程。最后,本文探讨了在保存卵巢组织生育力方面目前遇到的挑战和困难,同时也展望了未来的发展趋势,使其成为实施卵巢组织生育力保存的有价值参考。