Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan.
Graduate School of Pharmaceutical Sciences, Osaka University, Osaka 565-0871, Japan.
Cells. 2024 May 8;13(10):802. doi: 10.3390/cells13100802.
Infertility is considered a global health issue as it currently affects one in every six couples, with female factors reckoned to contribute to partly or solely 50% of all infertility cases. Over a thousand genes are predicted to be highly expressed in the female reproductive system and around 150 genes in the ovary. However, some of their functions in fertility remain to be elucidated. In this study, 13 ovary and/or oocyte-enriched genes (, , , , , , , , , , , , ) were individually knocked out by the CRISPR/Cas9 system. Mating tests showed that these 13 mutant mouse lines were capable of producing offspring. In addition, we observed the histology section of ovaries and performed in vitro fertilization in five mutant mouse lines. We found no significant anomalies in terms of ovarian development and fertilization ability. In this study, 13 different mutant mouse lines generated by CRISPR/Cas9 genome editing technology revealed that these 13 genes are individually not essential for female fertility in mice.
不孕不育被认为是一个全球性的健康问题，因为它目前影响着每六对夫妇中的一对，其中女性因素被认为导致了全部不孕不育病例的 50%或部分原因。据预测，有超过一千个基因在女性生殖系统中高度表达，而在卵巢中则有大约 150 个基因。然而，它们在生育能力方面的一些功能仍有待阐明。在这项研究中，我们通过 CRISPR/Cas9 系统分别敲除了 13 个卵巢和/或卵母细胞富集基因（,,,,,,,,,,,,, ）。交配测试表明，这 13 条突变小鼠品系能够产生后代。此外，我们观察了卵巢的组织学切片，并在 5 条突变小鼠品系中进行了体外受精。我们没有发现卵巢发育和受精能力方面的显著异常。在这项研究中，通过 CRISPR/Cas9 基因组编辑技术生成的 13 种不同的突变小鼠品系表明，这些基因单独对于小鼠的雌性生育能力并非必不可少。
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