College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China; Department of Biology, College of Science, Shantou University, Shantou 515063, Guangdong, PR China.
Department of Biology, College of Science, Shantou University, Shantou 515063, Guangdong, PR China.
Int J Biol Macromol. 2024 Jun;271(Pt 1):132578. doi: 10.1016/j.ijbiomac.2024.132578. Epub 2024 May 22.
Ulcerative colitis is a chronic, spontaneous inflammatory bowel disease that primarily affects the colon. This study aimed to explore how Porphyra haitanensis porphyran (PHP) modulates the immune response and the associated mechanisms that alleviate dextran sulphate sodium-induced colitis in mice. Histological assessments via H&E staining and AB-PAS staining revealed that PHP intervention partially restored the number of goblet cells and improved intestinal mucosal function. Immunohistochemical and Western blot analyses of claudin-1, occludin, and MUC-2 demonstrated that PHP could repair the intestinal barrier and reduce colon damage by upregulating the expression of these proteins. PHP intervention was associated with a decrease in pro-inflammatory cytokine expression and an increase in anti-inflammatory cytokine expression. Moreover, the expression of proteins involved in intestinal immune homing, such as CCR-9, CCL-25, MAdCAM-1, and α4β7, was significantly suppressed in response to PHP treatment. Conversely, PHP upregulates the expression of CD40 and TGF-β1, both of these can promote healing and reduce inflammation in the gut lining. This study demonstrates that PHP can ameliorate ulcerative colitis by enhancing the intestinal barrier and modulating immune responses. These findings offer valuable insights into the potential utility of P. haitanensis as a promising natural product for managing ulcerative colitis.
溃疡性结肠炎是一种慢性、自发性炎症性肠病，主要影响结肠。本研究旨在探讨紫菜岩藻聚糖（PHP）如何调节免疫反应以及相关机制，从而减轻葡聚糖硫酸钠诱导的小鼠结肠炎。通过 H&E 染色和 AB-PAS 染色进行组织学评估显示，PHP 干预部分恢复了杯状细胞数量，改善了肠道黏膜功能。Claudin-1、occludin 和 MUC-2 的免疫组化和 Western blot 分析表明，PHP 可以通过上调这些蛋白的表达来修复肠道屏障并减轻结肠损伤。PHP 干预与促炎细胞因子表达减少和抗炎细胞因子表达增加有关。此外，PHP 治疗还显著抑制了参与肠道免疫归巢的蛋白的表达，如 CCR-9、CCL-25、MAdCAM-1 和 α4β7。相反，PHP 上调了 CD40 和 TGF-β1 的表达，这两者都可以促进肠道内膜的愈合和减少炎症。本研究表明，PHP 通过增强肠道屏障和调节免疫反应来改善溃疡性结肠炎。这些发现为紫菜作为一种有前途的天然产物治疗溃疡性结肠炎提供了有价值的见解。
