Grassland Research Institute, Xinjiang Academy of Animal Sciences, Urumqi 830000, China.
College of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, China.
Genes (Basel). 2024 Apr 25;15(5):546. doi: 10.3390/genes15050546.
is a dominant desert shrub in Xinjiang, China, with high economic and ecological value. However, molecular systematics research on is lacking. To resolve the genetic composition of within Amaranthaceae and its systematic relationship with related genera, we used a second-generation Illumina sequencing system to detect the chloroplast genome of and analyze its assembly, annotation, and phylogenetics. Total length of the chloroplast genome of reached 152,287 bp, with 84 protein-coding genes, 36 tRNAs, and eight rRNAs. Codon usage analysis showed the majority of codons ending with base A/U. Mononucleotide repeats were the most common (85.42%) of the four identified simple sequence repeats. A comparison with chloroplast genomes of six other Amaranthaceae species indicated contraction and expansion of the inverted repeat boundary region in , with some genes (, , ) differing in length and distribution. Among the seven species, the variation in non-coding regions was greater. Phylogenetic analysis revealed , , , and to have a close monophyletic relationship. By sequencing the chloroplast genome, this research resolves the relatedness among 35 Amaranthaceae species, providing molecular insights for germplasm utilization, and theoretical support for studying evolutionary relationships.
是中国新疆的一种优势荒漠灌木,具有很高的经济和生态价值。然而,对 的分子系统学研究还很缺乏。为了解决苋科内 的遗传组成及其与相关属的系统关系,我们使用第二代 Illumina 测序系统检测了 的叶绿体基因组,并对其组装、注释和系统发育进行了分析。 的叶绿体基因组全长达到 152287bp,包含 84 个蛋白编码基因、36 个 tRNA 和 8 个 rRNA。密码子使用分析表明,大多数终止密码子为 A/U。单核苷酸重复是四种已识别的简单重复序列中最常见的(85.42%)。与其他六个苋科物种的叶绿体基因组比较表明, 在反向重复边界区域发生了收缩和扩张,一些基因( 、 、 )在长度和分布上存在差异。在这七个物种中,非编码区的变异更大。系统发育分析表明 、 、 、 和 具有密切的单系关系。通过测序 的叶绿体基因组,本研究解决了 35 种苋科物种之间的亲缘关系,为种质资源利用提供了分子见解,并为研究进化关系提供了理论支持。