Dujíčková Linda, Olexiková Lucia, Makarevich Alexander V, Bartková Alexandra Rosenbaum, Němcová Lucie, Chrenek Peter, Strejček František
Research Institute for Animal Production Nitra, National Agricultural and Food Centre (NPPC), Hlohovecká 2, 951 41 Lužianky, Slovakia.
Department of Botany and Genetics, Faculty of Natural Sciences and Informatics, Constantine the Philosopher University in Nitra, Tr. A. Hlinku 1, 949 01 Nitra, Slovakia.
Antioxidants (Basel). 2024 Apr 30;13(5):556. doi: 10.3390/antiox13050556.
Various antioxidants are tested to improve the viability and development of cryopreserved oocytes, due to their known positive health effects. The aim of this study was to find whether astaxanthin (AX), a xanthophyll carotenoid, could mitigate deteriorations that occurred during the vitrification/warming process in bovine oocytes. Astaxanthin (2.5 µM) was added to the maturation medium during the post-warm recovery period of vitrified oocytes for 3 h. Afterward, the oocytes were fertilized in vitro using frozen bull semen and presumptive zygotes were cultured in the B2 Menezo medium in a co-culture with BRL-1 cells at 38.5 °C and 5% CO until the blastocyst stage. AX addition significantly reduced ROS formation, lipid peroxidation, and lysosomal activity, while increasing mitochondrial activity in vitrified oocytes. Although the effect of AX on embryo development was not observed, it stimulated cell proliferation in the blastocysts derived from vitrified oocytes and improved their quality by upregulation or downregulation of some genes related to apoptosis (, ), oxidative stress (, ), and development () compared to the vitrified group without AX. Therefore, the antioxidant properties of astaxanthin even during short exposure to bovine vitrified/warmed oocytes resulted in improved blastocyst quality comparable to those from fresh oocytes.
由于各种抗氧化剂已知具有积极的健康效应，因此对其进行了测试，以提高冷冻保存的卵母细胞的活力和发育能力。本研究的目的是确定叶黄素类胡萝卜素虾青素（AX）是否可以减轻牛卵母细胞玻璃化/复温过程中发生的退化。在玻璃化卵母细胞复温后的恢复期，将虾青素（2.5µM）添加到成熟培养基中3小时。之后，使用冷冻的公牛精液对卵母细胞进行体外受精，并将推定的受精卵在B2 Menezo培养基中与BRL-1细胞共培养，在38.5°C和5%二氧化碳的条件下培养至囊胚阶段。添加虾青素可显著减少玻璃化卵母细胞中的活性氧形成、脂质过氧化和溶酶体活性，同时增加线粒体活性。虽然未观察到虾青素对胚胎发育的影响，但与未添加虾青素的玻璃化组相比，它刺激了玻璃化卵母细胞来源的囊胚中的细胞增殖，并通过上调或下调一些与细胞凋亡（、）、氧化应激（、）和发育（）相关的基因来改善其质量。因此，即使在短时间暴露于牛玻璃化/复温卵母细胞的情况下，虾青素的抗氧化特性也能提高囊胚质量，使其与新鲜卵母细胞相当。
