Wen Wei, Hu Miao, Gao Yaxin, Zhang Pengfei, Meng Weimin, Zhang Fengxia, Fan Bei, Wang Fengzhong, Li Shuying
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China.
Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Foods. 2024 May 14;13(10):1525. doi: 10.3390/foods13101525.
Microbial nitrogen sources are promising, and soy protein as a plant-based nitrogen source has absolute advantages in creating microbial culture medium in terms of renewability, eco-friendliness, and greater safety. Soy protein is rich in variety due to different extraction technologies and significantly different in the cell growth and metabolism of microorganisms as nitrogen source. Therefore, different soy proteins (soy meal powder, SMP; soy peptone, SP; soy protein concentrate, SPC; soy protein isolate, SPI; and soy protein hydrolysate, SPH) were used as nitrogen sources to culture , , and to evaluate the suitable soy nitrogen sources of the above strains. The results showed that had the highest bacteria density in SMP medium; had the highest bacteria density in SPI medium; and had the highest PMV in SPI medium. Nattokinase activity was the highest in SP medium; the bacteriostatic effect of nisin was the best in SPI medium; and the clavulanic acid concentration was the highest in SMP medium. Based on analyzing the correlation between the nutritional composition and growth metabolism of the strains, the results indicated that the protein content and amino acid composition were the key factors influencing the cell growth and metabolism of the strains. These findings present a new, high-value application opportunity for soybean protein.
微生物氮源前景广阔,大豆蛋白作为一种植物性氮源,在可再生性、生态友好性和更高安全性方面,对于制备微生物培养基具有绝对优势。由于提取技术不同,大豆蛋白种类丰富,作为氮源时对微生物细胞生长和代谢的影响差异显著。因此,选用不同的大豆蛋白(豆粕粉、大豆蛋白胨、大豆浓缩蛋白、大豆分离蛋白和大豆蛋白水解物)作为氮源培养[具体菌株名称未给出],以评估上述菌株适宜的大豆氮源。结果表明,[具体菌株名称未给出]在豆粕粉培养基中的细菌密度最高;[具体菌株名称未给出]在大豆分离蛋白培养基中的细菌密度最高;[具体菌株名称未给出]在大豆分离蛋白培养基中的PMV最高。纳豆激酶活性在大豆蛋白胨培养基中最高;乳酸链球菌素的抑菌效果在大豆分离蛋白培养基中最佳;棒酸浓度在豆粕粉培养基中最高。通过分析菌株营养成分与生长代谢之间的相关性,结果表明蛋白质含量和氨基酸组成是影响菌株细胞生长和代谢的关键因素。这些发现为大豆蛋白提供了一个新的高价值应用机会。