Li Wenhui, Zhang Yinxiao, Li He, Zhang Chi, Zhang Jian, Uddin Jalal, Liu Xinqi
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University Beijing 100048 China
RSC Adv. 2020 Apr 29;10(28):16737-16748. doi: 10.1039/d0ra01632b. eCollection 2020 Apr 23.
Soybean protein (Pro) and soybean oligopeptide (Pep) were subjected to simulated digestion to study the effect of Pep on the growth and metabolism of JCM 1132. First, the molecular weight distribution differences of samples before and after digestion were compared, and the samples were used to replace the nitrogen source components in the culture media. Then, the viable cell numbers, lactic acid and acetic acid content, differential metabolites, and metabolic pathways during the culturing process were measured. Results showed that the digested soybean oligopeptide (dPep) was less efficient than MRS medium in promoting the growth, but by increasing the content of the intermediates during the tricarboxylic acid (TCA) cycle, its metabolic capacity was significantly improved. Besides, due to the low molecular weight of dPep, it can be better transported and utilized. And dPep significantly strengthened the amino acid metabolism and weakened the glycerol phospholipid metabolism, so the ability of dPep in promoting the growth and metabolism of JCM 1132 is higher than the digested soybean protein (dPro).
对大豆蛋白（Pro）和大豆寡肽（Pep）进行模拟消化，以研究Pep对JCM 1132生长和代谢的影响。首先，比较消化前后样品的分子量分布差异，并将样品用于替代培养基中的氮源成分。然后，测定培养过程中的活菌数、乳酸和乙酸含量、差异代谢物及代谢途径。结果表明，消化后的大豆寡肽（dPep）在促进生长方面不如MRS培养基有效，但通过增加三羧酸（TCA）循环中间产物的含量，其代谢能力显著提高。此外，由于dPep分子量较低，其转运和利用效果更好。并且dPep显著增强了氨基酸代谢，减弱了甘油磷脂代谢，因此dPep促进JCM 1132生长和代谢的能力高于消化后的大豆蛋白（dPro）。
