Xavier Lorena Soares, Pacheco Flaviana Coelho, Nalon Gabriela Aparecida, Cunha Jeferson Silva, Santos Fabio Ribeiro Dos, Pacheco Ana Flávia Coelho, Tribst Alline Artigiani Lima, Leite Júnior Bruno Ricardo de Castro
Department of Food Technology (DTA), Federal University of Viçosa (UFV), Viçosa 36570-900, MG, Brazil.
Instituto de Laticínios Cândido Tostes, Empresa Agropecuária de Minas Gerais (EPAMIG), Tenente Luiz de Freitas, 116, Juiz de Fora 36045-560, MG, Brazil.
Foods. 2024 May 16;13(10):1558. doi: 10.3390/foods13101558.
This work investigated the fermentation kinetics and characteristics of goat yogurt supplemented with bovine whey protein isolate (WPI) (0%, 2.5% and 5.0%) subjected to high shear dispersion (HSD) assisted by ultrasound (US). Protein supplementation and the physical processes increased the electronegativity of the zeta potential (≤60%), whereas particle size reduction was observed only with physical processes (≤42%). The addition of 2.5% WPI reduced yogurt fermentation time by 30 min. After 24 h of storage at 7 °C, lactic acid bacteria counts did not differ between samples (≥8 log CFU/mL), and the supplementation was sufficient to increase the apparent viscosity (≤5.65 times) and water-holding capacity (WHC) of the yogurt (≤35% increase). However, supplementation combined with physical processes promoted greater improvements in these parameters (6.41 times in apparent viscosity and 48% in WHC) ( < 0.05), as confirmed by the denser and better-organized protein clusters observed in microscopic evaluation. Thus, both approaches proved to be promising alternatives to improve goat yogurt quality. Therefore, the decision to adopt these strategies, either independently or in combination, should consider cost implications, the product quality, and market demand.
本研究调查了添加不同比例(0%、2.5%和5.0%)乳清蛋白分离物(WPI)的山羊酸奶在超声(US)辅助高剪切分散(HSD)作用下的发酵动力学和特性。蛋白质添加和物理处理提高了zeta电位的负电性(≤60%),而仅物理处理可使粒径减小(≤42%)。添加2.5%WPI可使酸奶发酵时间缩短30分钟。在7℃储存24小时后,各样本间乳酸菌计数无差异(≥8 log CFU/mL),且该添加量足以提高酸奶的表观粘度(≤5.65倍)和持水能力(WHC)(≤35%)。然而,添加与物理处理相结合可使这些参数得到更大改善(表观粘度提高6.41倍,WHC提高48%)(P<0.05),微观评估中观察到的更致密、组织更好的蛋白质簇证实了这一点。因此,两种方法均被证明是改善山羊酸奶品质的有前景的替代方法。因此,决定单独或联合采用这些策略时,应考虑成本影响、产品质量和市场需求。