Zhejiang Chinese Medical University, Hangzhou 310053, China.
Molecules. 2024 May 14;29(10):2300. doi: 10.3390/molecules29102300.
Yinhua Pinggan Granule (YPG) is an approved compounded traditional Chinese medicine (TCM) prescription for the treatment of cold, cough, viral pneumonia, and related diseases. Due to its complicated chemical composition, the material basis of YPG has not been systematically investigated. In this study, an analytical method based on high-performance liquid chromatography (HPLC) coupled with Q-Exactive mass spectrometry was established. Together with the help of a self-built compound database and Compound Discoverer software 3.1, the chemical components in YPG were tentatively identified. Subsequently, six main components in YPG were quantitatively characterized with a high-performance liquid chromatography-diode array detector (HPLC-DAD) method. As a result, 380 components were annotated, including 19 alkaloids, 8 organic acids, 36 phenolic acids, 27 other phenols, 114 flavonoids, 75 flavonoid glycoside, 72 terpenes, 11 anthraquinones, and 18 other compounds. Six main components, namely, chlorogenic acid, puerarin, 3'-methoxypuerarin, polydatin, glycyrrhizic acid, and emodin, were quantified simultaneously. The calibration curves of all six analytes showed good linearity (R > 0.9990) within the test ranges. The precision, repeatability, stability, and recovery values were all in acceptable ranges. In addition, the total phenol content and DPPH scavenging activity of YPG were also determined. The systematic elucidation of the chemical components in YPG in this study may provide clear chemical information for the quality control and pharmacological research of YPG and related TCM compounded prescriptions.
银花平肝颗粒（YPG）是一种已批准的复方中药（TCM）处方，用于治疗感冒、咳嗽、病毒性肺炎及相关疾病。由于其复杂的化学成分，YPG 的物质基础尚未得到系统研究。本研究建立了一种基于高效液相色谱（HPLC）结合 Q-Exactive 质谱的分析方法。同时借助自建的化合物数据库和 Compound Discoverer 软件 3.1，对 YPG 中的化学成分进行了初步鉴定。随后，采用高效液相色谱-二极管阵列检测器（HPLC-DAD）法对 YPG 中的 6 个主要成分进行定量分析。结果注释了 380 个成分，包括 19 个生物碱、8 个有机酸、36 个酚酸、27 个其他酚类、114 个黄酮类、75 个黄酮苷类、72 个萜类、11 个蒽醌类和 18 个其他化合物。同时对 6 个主要成分，即绿原酸、葛根素、3'-甲氧基葛根素、虎杖苷、甘草酸和大黄素，进行了同时定量分析。所有 6 种分析物的校准曲线均呈良好的线性（R > 0.9990）。精密度、重复性、稳定性和回收率的测定值均在可接受范围内。此外，还测定了 YPG 的总酚含量和 DPPH 清除活性。本研究对 YPG 中化学成分的系统阐明，可为 YPG 及相关 TCM 复方制剂的质量控制和药理研究提供明确的化学信息。
