Fukushima Takeshi, Koishi Mikoto, Sakamoto Tatsuya, Onozato Mayu
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi 274-8510, Chiba, Japan.
Molecules. 2024 May 16;29(10):2341. doi: 10.3390/molecules29102341.
Mixed-mode high-performance liquid chromatography (HPLC) is increasingly used for the analysis of ionic and highly hydrophilic drugs, which are difficult to separate by conventional single-mode HPLC. In the former case, chromatographic separation is achieved using one of the several commercially available mixed-mode stationary phases, typically combinations of reversed and ion-exchange phases. Moreover, mixed-mode stationary phases can be used as solid-phase extraction (SPE) sorbents. This review focuses on the recent applications of mixed-mode stationary phases in the chromatographic analysis of bioactive compounds, such as drugs, herbicides, and pesticides. Specifically, we briefly summarize HPLC methods utilizing mixed-mode stationary phases and SPE pretreatment procedures utilizing mixed-mode sorbents developed in the last decade, thus providing a reference work for overcoming the difficulties in analyzing ionized or hydrophilic drugs by conventional reversed-phase chromatography.
混合模式高效液相色谱法（HPLC）越来越多地用于分析离子型和高亲水性药物，这些药物难以通过传统的单模式HPLC进行分离。在前一种情况下，使用几种市售混合模式固定相之一实现色谱分离，通常是反相和离子交换相的组合。此外，混合模式固定相可用作固相萃取（SPE）吸附剂。本综述重点关注混合模式固定相在生物活性化合物（如药物、除草剂和杀虫剂）色谱分析中的最新应用。具体而言，我们简要总结了利用混合模式固定相的HPLC方法以及过去十年中开发的利用混合模式吸附剂的SPE预处理程序，从而为克服传统反相色谱法分析离子化或亲水性药物的困难提供参考依据。
