采用混合模式色谱法拆分维加特林对映异构体及其在大鼠血浆中维加特林对映异构体水平测定中的应用。
Separation of vigabatrin enantiomers using mixed-mode chromatography and its application to determine the vigabatrin enantiomer levels in rat plasma.
机构信息
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba 274-8510, Japan.
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba 274-8510, Japan; Department of Clinical Pharmacy, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba 274-8510, Japan.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Aug 1;1179:122866. doi: 10.1016/j.jchromb.2021.122866. Epub 2021 Jul 27.
Mixed-mode chromatography-comprising a mixed phase with reversed and ionic phases, enabling hydrophobic and ion-exchange interactions simultaneously-was applied to identify vigabatrin enantiomers by HPLC with pre-column fluorescence derivatization with 2,5-dioxopyrrolidin-1-yl (4-(((2-nitrophenyl)sulfonyl)oxy)-6-(3-oxomorpholino)quinoline-2-carbonyl)prolinate (Ns-MOK-(S)-Pro-OSu). The MOK-(S)-Pro-vigabatrin enantiomers were efficiently separated within 12 min (total analysis time per sample: 28 min, including washing and equilibrium time for the column). The mobile phase was HO/CHOH/10 mM ammonium formate (pH 2.0) (20/20/60, v/v/v). Column temperature was maintained at 60℃. The proposed HPLC method could successfully monitor plasma vigabatrin enantiomer levels in rats administered (±)-vigabatrin (50 mg/kg, p.o.).
混合模式色谱-包括反相和离子相的混合相，能够同时实现疏水性和离子交换相互作用-被应用于通过 HPLC 用柱前荧光衍生化 2,5-二氧代吡咯烷-1-基（4-（（2-硝基苯基）磺酰基）氧基）-6-（3-氧代吗啉代）喹啉-2-甲酰基（Ns-MOK-（S）-Pro-OSu）来鉴定氨己烯酸对映异构体。MOK-（S）-Pro-氨己烯酸对映异构体在 12 分钟内得到有效分离（每个样品的总分析时间：28 分钟，包括柱的洗涤和平衡时间）。流动相为 HO/CHOH/10mM 甲酸铵（pH 2.0）（20/20/60，v/v/v）。柱温保持在 60℃。所提出的 HPLC 方法可成功监测给予（±）-氨己烯酸（50mg/kg，po）的大鼠的血浆氨己烯酸对映异构体水平。
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