牛胸腺提取物（胸腺刺激素）对脐血淋巴细胞中T细胞分化的体外作用。

In vitro effect of a bovine thymic extract (thymostimulin) on T-cell differentiation in cord blood lymphocytes.

作者信息

Lin C Y, Chu C M

出版信息

Pediatr Pharmacol (New York). 1985;5(3):181-7.

Abstract

Cord blood lymphocytes were isolated from 40 normal newborns. The initial 20 samples were used to determine the dose-response curve of bovine thymic extract (Thymostimulin) by the measurement of active T cells. Results showed that the active T cells increased significantly when the Thymostimulin concentration was increased to 50 ng/ml and 100 ng/ml. The remaining 20 samples were divided into three portions and preincubated either with 50 ng and 100 ng of Thymostimulin or without Thymostimulin. The total T cells, active T cells, B cells, T-cell subsets, and lymphoproliferative responses to phytohemagglutinin, concanavalin A, and pokeweed mitogen were determined. The results showed that the active T cells, total T cells, B cells, OKT4 cells, and OKT8 cells were significantly increased after Thymostimulin treatment. The lymphoproliferative responses were also significantly increased. These data strongly support our conclusion that Thymostimulin has a marked stimulating effect on human lymphocyte proliferation and differentiation.

摘要

从40名正常新生儿中分离出脐血淋巴细胞。最初的20个样本用于通过测量活性T细胞来确定牛胸腺提取物（胸腺刺激素）的剂量反应曲线。结果显示，当胸腺刺激素浓度增加到50 ng/ml和100 ng/ml时，活性T细胞显著增加。其余20个样本分为三份，分别与50 ng和100 ng的胸腺刺激素预孵育或不使用胸腺刺激素预孵育。测定了总T细胞、活性T细胞、B细胞、T细胞亚群以及对植物血凝素、刀豆球蛋白A和商陆有丝分裂原的淋巴细胞增殖反应。结果显示，胸腺刺激素处理后，活性T细胞、总T细胞、B细胞、OKT4细胞和OKT8细胞显著增加。淋巴细胞增殖反应也显著增加。这些数据有力地支持了我们的结论，即胸腺刺激素对人淋巴细胞的增殖和分化有显著的刺激作用。