Clark R J, Tschida A R, Markowitz H
J Clin Lab Immunol. 1985 Dec;18(4):191-4.
Analysis of human serum for total hemolytic complement (CH50) is useful in following the course of patients who have diseases such as lupus nephritis or hypocomplementemic glomerulonephritis. A semi-automated system has been devised that facilitates test performance and reduces cost. An Autoprep dilutor is driven by an Apple IIe microprocessor. Samples are picked up, diluted with buffer, and added to a 96-well flat-bottom Linbro plate in a final volume of 100 microliters. Sensitized sheep RBC (100 microliters) are then added to each well. The plate is incubated at 37 degrees C for 90 min, agitated on a shaker, and then read by a Dynatech MR600 plate reader at 630 nm. The microprocessor converts the reading to percentage hemolysis by utilizing both a buffer blank and a complete hemolysis blank (saponin); these values are applied to the von Krogh equation to yield a CH50 value. Values obtained for normal human serum samples correlate well with those from an established semi-automated method (Technicon) in use in our laboratory.
对人血清进行总溶血补体（CH50）分析，有助于跟踪患有狼疮性肾炎或低补体血症性肾小球肾炎等疾病患者的病程。现已设计出一种半自动系统，该系统便于进行检测并降低成本。自动稀释仪由Apple IIe微处理器驱动。吸取样品，用缓冲液稀释，然后加入到96孔平底林伯板中，最终体积为100微升。然后向每个孔中加入致敏绵羊红细胞（100微升）。将平板在37℃孵育90分钟，在振荡器上振荡，然后用Dynatech MR600酶标仪在630nm处读取。微处理器通过使用缓冲液空白和完全溶血空白（皂角苷）将读数转换为溶血百分比；这些值应用于冯·克罗格方程以得出CH50值。从正常人血清样品获得的值与我们实验室使用的既定半自动方法（Technicon）得到的值相关性良好。
