Sanya Institute of Nanjing Agricultural University, MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Physiology & Biochemistry, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Shandong Laboratory of Advanced Biomaterials and Medical Devices in Weihai, Weihai 264200, China.
J Hazard Mater. 2024 Jul 15;473:134680. doi: 10.1016/j.jhazmat.2024.134680. Epub 2024 May 22.
Due to the bacteria resistant to various first-line antibiotics, it is urgent to develop efficient antibiotic alternatives and formulate multidimensional strategies. Herein, supramolecular Chinese medicine nanoparticles are synthesized by self-assembly of berberine (BBR) and chlorogenic acid (CGA), which exhibit higher inhibitory effect against Staphylococcus aureus and multidrug-resistant Staphylococcus aureus (MRSA) than ampicillin, oxacillin, BBR, CGA, as well as mixture of BBR and CGA (minimum inhibitory concentration, MIC = 1.5 µM). The inhibition by BBR/CGA nanoparticles (2.5 µM) reaches 99.06 % for MRSA, which is significantly higher than ampicillin (29.03 %). The nanoparticles with 1/2 MIC can also synergistically restore the antimicrobial activity of ampicillin against MRSA. Moreover, in vivo therapeutic outcome in the murine skin wound infection model suggests that the nanoparticles are able to promote wound healing. This study provides new insights in the application of Chinese medicines self-assembly for MRSA inhibition, as well as solutions for potential persistent clinical infections and drug deficiencies.
由于各种一线抗生素耐药的细菌，迫切需要开发有效的抗生素替代品，并制定多维策略。在此，通过小檗碱（BBR）和绿原酸（CGA）的自组装合成了超分子中药纳米粒子，其对金黄色葡萄球菌和耐甲氧西林金黄色葡萄球菌（MRSA）的抑制作用高于氨苄西林、苯唑西林、BBR、CGA 以及 BBR 和 CGA 的混合物（最小抑菌浓度，MIC=1.5µM）。BBR/CGA 纳米粒子（2.5µM）对 MRSA 的抑制率达到 99.06%，明显高于氨苄西林（29.03%）。1/2 MIC 的纳米粒子还可以协同恢复氨苄西林对 MRSA 的抗菌活性。此外，在小鼠皮肤创伤感染模型中的体内治疗结果表明，纳米粒子能够促进创伤愈合。本研究为中药自组装在抑制 MRSA 中的应用提供了新的见解，也为潜在的持续性临床感染和药物短缺提供了解决方案。
