Section of Pharmaceutical Technology, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimioupolis Zografou, Athens 15771, Greece.
Centre of Polymer and Carbon Materials, Polish Academy of Sciences, Zabrze 41-819, Poland.
Langmuir. 2024 Jun 11;40(23):11936-11946. doi: 10.1021/acs.langmuir.4c00278. Epub 2024 May 26.
Lipid/copolymer colloidal systems are deemed hybrid materials with unique properties and functionalities. Their hybrid nature leads to complex interfacial phenomena, which have not been fully encoded yet, navigating their properties. Moving toward in-depth knowledge of such systems, a comprehensive investigation of them is imperative. In the present study, hybrid lipid/copolymer structures were fabricated and examined by a gamut of techniques, including dynamic light scattering, fluorescence spectroscopy, cryogenic transmission electron microscopy, microcalorimetry, and high-resolution ultrasound spectroscopy. The biomaterials that were mixed for this purpose at different ratios were 1,2-dioctadecanoyl--glycero-3-phosphocholine and four different linear, statistical (random) amphiphilic copolymers, consisting of oligo(ethylene glycol) methyl ether methacrylate as the hydrophilic comonomer and lauryl methacrylate as the hydrophobic one. The colloidal dispersions were studied for lipid/copolymer interactions regarding their physicochemical, morphological, and biophysical behavior. Their membrane properties and interactions with serum proteins were also studied. The aforementioned techniques confirmed the hybrid nature of the systems and the location of the copolymer in the structure. More importantly, the random architecture of the copolymers, the hydrophobic-to-hydrophilic balance of the nanoplatforms, and the lipid-to-polymer ratio are highlighted as the main design-influencing factors. Elucidating the lipid/copolymer interactions would contribute to the translation of hybrid nanoparticle performance and, thus, their rational design for multiple applications, including drug delivery.
脂质/共聚物胶体系统被认为是具有独特性质和功能的混合材料。它们的混合性质导致了复杂的界面现象，这些现象尚未被完全编码，从而影响了它们的性质。为了深入了解这些系统，对它们进行全面的研究是至关重要的。在本研究中，通过一系列技术制备和研究了混合脂质/共聚物结构，包括动态光散射、荧光光谱学、低温透射电子显微镜、微量热法和高分辨率超声光谱学。为此目的混合的生物材料以不同的比例混合，包括 1,2-二油酰基-甘油-3-磷酸胆碱和四种不同的线性、统计（随机）两亲共聚物，由聚乙二醇甲基醚甲基丙烯酸酯作为亲水单体和月桂基甲基丙烯酸酯作为疏水单体组成。研究了胶体分散体在物理化学、形态和生物物理行为方面的脂质/共聚物相互作用。还研究了它们的膜性质和与血清蛋白的相互作用。上述技术证实了系统的混合性质以及共聚物在结构中的位置。更重要的是，共聚物的随机结构、纳米平台的亲疏水性平衡以及脂质与聚合物的比例被强调为主要的设计影响因素。阐明脂质/共聚物的相互作用将有助于转化混合纳米颗粒的性能，从而为包括药物输送在内的多种应用进行合理的设计。
