Noskov A N, Ezepchuk Iu V
Mol Gen Mikrobiol Virusol. 1985 Jan(1):25-9.
The lymphocyte membranes from rabbit thymus were shown to bind specifically the staphylococcal enterotoxin type A (SEA). The glycolipid components were demonstrated to be absent from the SEA receptor complex on the surface of T-lymphocytes. The mild conditions were elaborated for the receptor membrane fraction solubilization by triton X-100. The affinity chromatography method was used to isolate the SEA binding membrane fraction, the major component of which is a protein with a 42,000 mol mass. The isolated preparation inhibits the specific binding of [125I]-SEA on the cellular (by T-lymphocytes) and subcellular (by membranes) levels.
已证明来自兔胸腺的淋巴细胞膜能特异性结合A型葡萄球菌肠毒素（SEA）。已证实T淋巴细胞表面的SEA受体复合物中不存在糖脂成分。阐述了用曲拉通X-100溶解受体膜部分的温和条件。采用亲和层析法分离SEA结合膜部分，其主要成分是一种分子量为42,000的蛋白质。分离出的制剂在细胞（由T淋巴细胞）和亚细胞（由膜）水平上抑制[125I]-SEA的特异性结合。
