[Isolation and characteristics of membrane fraction of rabbit T lymphocytes specifically binding the staphylococcal enterotoxin type A].

The lymphocyte membranes from rabbit thymus were shown to bind specifically the staphylococcal enterotoxin type A (SEA). The glycolipid components were demonstrated to be absent from the SEA receptor complex on the surface of T-lymphocytes. The mild conditions were elaborated for the receptor membrane fraction solubilization by triton X-100. The affinity chromatography method was used to isolate the SEA binding membrane fraction, the major component of which is a protein with a 42,000 mol mass. The isolated preparation inhibits the specific binding of [125I]-SEA on the cellular (by T-lymphocytes) and subcellular (by membranes) levels.