Department of Urology, Guangdong Medical University, Zhanjiang, China.
Department of Urology, Yuebei people's hospital, Shaoguan, China.
Kaohsiung J Med Sci. 2024 Jul;40(7):660-670. doi: 10.1002/kjm2.12845. Epub 2024 May 27.
Bladder cancer (BC) poses high morbidity and mortality, with urinary exosomal microRNA (miR)-21 showing potential value in its diagnosis and prognosis, and we probed its specific role. We prospectively selected 116 BC patients and 116 healthy volunteers as the BC and control groups, respectively. BC urinary exosomal miR-146a-5p, miR-93-5p, miR-663b, miR-21, and miR-4454 relative expression levels were assessed. The correlations between clinical indexes and urinary exosomal miR-21, prognostic value of miR-21, and diagnostic value of the five candidate miRNAs, urine cytology, and miRNA joint diagnostic panel for BC and urinary exosomal miR-21, miR-4454, and urine cytology for Ta-T1 and T2-T4 stage BC were analyzed. Urinary exosomal miR-146a-5p, miR-93-5p, miR-663b, miR-21, and miR-4454 were highly expressed in BC patients. miR-146a-5p, miR-93-5p, miR-663b, miR-21, miR-4454, miRNA combined diagnostic panel, and urine cytology had certain diagnostic value for BC, with miR-21, miR-4454, and miRNA co-diagnostic panel showing the highest diagnostic value. Collectively, urinary exosomal miR-21 was closely related to Tumor-Node-Metastasis staging and grading in BC patients. Urinary exosomal miR-21 had high diagnostic value for BC and Ta-T1 and T2-T4 stage BC, and had high predictive value for BC poor prognosis, providing an effective indicator for the occurrence, development, and prognostic assessment of BC.
膀胱癌（BC）发病率和死亡率高，尿外泌体 microRNA（miR）-21 在其诊断和预后中有潜在价值，本研究旨在探究其具体作用。前瞻性选择 116 例 BC 患者和 116 例健康志愿者分别作为 BC 组和对照组，评估 BC 患者尿外泌体 miR-146a-5p、miR-93-5p、miR-663b、miR-21 和 miR-4454 的相对表达水平。分析临床指标与尿外泌体 miR-21 的相关性、miR-21 的预后价值、5 个候选 miRNA、尿细胞学和 miRNA 联合诊断.panel 对 BC 的诊断价值，以及尿外泌体 miR-21、miR-4454 和尿细胞学对 Ta-T1 和 T2-T4 期 BC 的诊断价值。BC 患者的尿外泌体 miR-146a-5p、miR-93-5p、miR-663b、miR-21 和 miR-4454 表达水平均升高。miR-146a-5p、miR-93-5p、miR-663b、miR-21、miR-4454、miRNA 联合诊断 panel 和尿细胞学对 BC 具有一定的诊断价值，其中 miR-21、miR-4454 和 miRNA 联合诊断 panel 的诊断价值最高。综上所述，BC 患者尿外泌体 miR-21 与肿瘤-淋巴结-转移分期和分级密切相关。尿外泌体 miR-21 对 BC 及 Ta-T1 和 T2-T4 期 BC 具有较高的诊断价值，对 BC 预后不良具有较高的预测价值，为 BC 的发生、发展和预后评估提供了有效的指标。
