Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (ICS-CAAS), Beijing 100081, China.
Plant Dis. 2024 Oct;108(10):3044-3051. doi: 10.1094/PDIS-04-24-0859-RE. Epub 2024 Sep 16.
Powdery mildew caused by DC is a global notorious disease on peas. Deploying resistance pea cultivars is the most efficient and environmentally friendly method for disease control. This study focuses on revealing the resistance genes in three pea germplasms and developing their functional markers for resistance breeding. The identification of resistance genes involved genetic mapping and the sequencing of the pea mildew resistance locus O homolog gene. To confirm the heredity of three resistant germplasms, they were crossed with susceptible cultivars to generate F, F, and F populations. The F generation exhibited susceptibility to , whereas the segregation patterns in subsequent generations adhered to the 3:1 (susceptible: resistant) and 1:2:1 (susceptible homozygotes: heterozygotes: resistant homozygotes) ratios, indicating that powdery mildew resistance was governed by a single recessive gene in each germplasm. Analysis of -linked markers and genetic mapping suggested that the resistance genes could be alleles in these germplasms. The multiple clone sequencing results of the three homologous genes showed they were novel alleles, named -15, -16, and -17. The -15 and -16 were caused by 1-bp deletion at position 335 (A) and 429 (T) in exon 3, respectively, whereas -17 was caused by a 1-bp insertion at position 248 in exon 3, causing a frame-shift mutation and premature termination of protein translation. Their respective functional markers, kompetitive allele-specific PCR (KASP)--15, KASP--16, and KASP--17, were successfully developed and validated in respective mapping populations and pea germplasms. These results provide valuable tools for pea breeding resistance to . .
由 DC 引起的白粉病是豌豆上的一种全球性严重病害。利用具有抗性的豌豆品种是控制病害最有效和最环保的方法。本研究重点揭示了三个豌豆种质资源中的抗性基因，并开发了它们的功能标记用于抗性育种。抗性基因的鉴定涉及遗传图谱的构建和豌豆白粉病抗性位点 O 同源基因的测序。为了确认三个抗性种质的遗传特性，它们与易感品种进行杂交，产生了 F、F 和 F 群体。F 代对表现出易感性，而随后几代的分离模式符合 3:1（易感：抗性）和 1:2:1（易感纯合子：杂合子：抗性纯合子）的比例，表明白粉病抗性在每个种质中由单个隐性基因控制。-连锁标记和遗传图谱分析表明，这些种质中的抗性基因可能是 等位基因。三个同源 基因的多克隆测序结果表明，它们是新的 等位基因，分别命名为-15、-16 和-17。-15 和 -16 分别是由于 3 号外显子 335 位（A）和 429 位（T）的 1-bp 缺失引起的，而-17 是由于 3 号外显子 248 位的 1-bp 插入引起的，导致 蛋白翻译的移码突变和提前终止。它们各自的功能标记，竞争性等位基因特异性 PCR（KASP）--15、KASP--16 和 KASP--17，在各自的图谱群体和豌豆种质中成功开发和验证。这些结果为豌豆对白粉病的抗性育种提供了有价值的工具。
