L(+)-抗坏血酸跨二棕榈酰磷脂酰胆碱囊泡膜的扩散。

The diffusion of L(+)-ascorbic acid across DPPC vesicle membranes.

作者信息

Sapper H, Röth K D, Lohmann W

机构信息

Institut für Biophysik, Justus Liebig Universität Giessen, F.R. Germany.

出版信息

J Microencapsul. 1985 Jan-Mar;2(1):23-30. doi: 10.3109/02652048509049574.

DOI:10.3109/02652048509049574

PMID:3880475

Abstract

The transmembrane diffusion of L(+)-ascorbic acid has been studied by means of 1H-n.m.r. spectroscopy using small unilamellar DPPC vesicles as a model system. It is shown that the electro-neutral form of L(+)-ascorbic acid diffuses faster across the membranes than the anionic form by about two orders of magnitude. The diffusion is influenced by a molecular interaction between L(+)-ascorbate and the membrane surface and depends also on the fluidity of the membrane. The calculated permeation coefficients of neutral L(+)-ascorbic acid are between 6 x 10(-10) and 3 x 10(-8) cm s-1 (35-52 degrees C).

摘要

利用小单层二棕榈酰磷脂酰胆碱（DPPC）囊泡作为模型系统，通过1H核磁共振光谱法研究了L(+)-抗坏血酸的跨膜扩散。结果表明，L(+)-抗坏血酸的电中性形式在膜上的扩散速度比阴离子形式快约两个数量级。这种扩散受到L(+)-抗坏血酸盐与膜表面之间分子相互作用的影响，并且还取决于膜的流动性。中性L(+)-抗坏血酸的计算渗透系数在6×10(-10)至3×10(-8) cm s-1之间（35 - 52摄氏度）。