Marucci Center for Blueberry Cranberry Research and Extension, Rutgers, the State University of NJ, Chatsworth, NJ, USA.
Complete Phytochemical Solutions, LLC, Cambridge, WI, USA.
J Diet Suppl. 2024;21(5):633-659. doi: 10.1080/19390211.2024.2356592. Epub 2024 May 28.
Urinary tract infection (UTI) prevention benefits of cranberry intake are clinically validated, especially for women and children. To ensure the benefits of cranberry dietary supplement products, the anti-adhesion activity (AAA) against uropathogenic bacteria is routinely used in in vitro bioassays to determine the activity in whole product formulations, isolated compounds, and ex vivo bioassays to assess urinary activity following intake. D-mannose is another dietary supplement taken for UTI prevention, based on the anti-adhesion mechanism.
Compare the relative AAA of cranberry and D-mannose dietary supplements against the most important bacterial types contributing to the pathogenesis of UTI, and consider how certain components potentially induce in vivo activity.
The current study used a crossover design to determine ex vivo AAA against both P- and Type 1-fimbriated uropathogenic of either D-mannose or a cranberry fruit juice dry extract product containing 36 mg of soluble proanthocyanidins (PACs), using bioassays that measure urinary activity following consumption. AAA of extracted cranberry compound fractions and D-mannose were compared in vitro and potential induction mechanisms of urinary AAA explored.
The cranberry dietary supplement exhibited both P-type and Type 1 in vitro and ex vivo AAA, while D-mannose only prevented Type 1 adhesion. Cranberry also demonstrated more robust and consistent ex vivo urinary AAA than D-mannose over each 1-week study period at different urine collection time points. The means by which the compounds with in vitro activity in each supplement product could potentially induce the AAA in urines was discussed relative to the data.
Results of the current study provide consumers and healthcare professionals with additional details on the compounds and mechanisms involved in the positive, broad-spectrum AAA of cranberry against both bacterial types most important in UTIs and uncovers limitations on AAA and effectiveness of D-mannose compared to cranberry.
蔓越莓摄入对预防尿路感染（UTI）有临床验证的益处，尤其适用于女性和儿童。为了确保蔓越莓膳食补充剂产品的益处，抗粘附活性（AAA）常用于体外生物测定以确定整个产品配方、分离化合物和体外生物测定中的活性，以评估摄入后的尿液活性。基于抗粘附机制，D-甘露糖也是一种用于预防 UTI 的膳食补充剂。
比较蔓越莓和 D-甘露糖膳食补充剂对导致 UTI 发病机制的最重要细菌类型的相对 AAA，并考虑某些成分如何潜在地诱导体内活性。
本研究使用交叉设计来确定针对 D-甘露糖或含有 36 毫克可溶原花青素（PACs）的蔓越莓果汁干提取物产品的 P-型和 1 型菌毛尿路致病性细菌的体外和体外 AAA，使用生物测定来测量消耗后尿液中的活性。比较了蔓越莓化合物提取物和 D-甘露糖的体外 AAA，并探讨了潜在的诱导尿液 AAA 的机制。
蔓越莓膳食补充剂在体外和体内均表现出 P 型和 1 型 AAA，而 D-甘露糖仅能预防 1 型粘附。在每个为期 1 周的研究期间，在不同的尿液采集时间点，蔓越莓的体外和体内尿液 AAA 均比 D-甘露糖更强大且更一致。还讨论了每种补充剂产品中具有体外活性的化合物如何通过数据潜在地诱导尿液中的 AAA。
本研究的结果为消费者和医疗保健专业人员提供了有关参与蔓越莓对两种在 UTI 中最重要的细菌类型的广谱积极 AAA 的化合物和机制的更多详细信息，并揭示了与蔓越莓相比 D-甘露糖的 AAA 和有效性的局限性。
