Faculty of Hepato-Pancreato-Biliary Surgery, Institute of Hepatobiliary Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing, 100853, China; School of Basic Medicine, Inner Mongolia Medical University, Hohhot, China.
Faculty of Hepato-Pancreato-Biliary Surgery, Institute of Hepatobiliary Surgery, The First Medical Center, Chinese PLA General Hospital, Beijing, 100853, China.
Virology. 2024 Aug;596:110125. doi: 10.1016/j.virol.2024.110125. Epub 2024 May 23.
Influenza viruses present a significant threat to global health. The production of a universal vaccine is considered essential due to the ineffectiveness of current seasonal influenza vaccines against mutant strains. mRNA technology offers new prospects in vaccinology, with various candidates for different infectious diseases currently in development and testing phases. In this study, we encapsulated a universal influenza mRNA vaccine. The vaccine encoded influenza hemagglutinin (HA), nucleoprotein (NP), and three tandem repeats of matrix protein 2 (3M2e). Twice-vaccinated mice exhibited strong humoral and cell-mediated immune responses in vivo. Notably, these immune responses led to a significant reduction in viral load of the lungs in challenged mice, and also conferred protection against future wild-type H1N1, H3N2, or H5N1 influenza virus challenges. Our findings suggest that this mRNA-universal vaccine strategy for influenza virus may be instrumental in mitigating the impact of future influenza pandemics.
流感病毒对全球健康构成重大威胁。由于当前季节性流感疫苗对突变株无效，因此生产通用疫苗被认为至关重要。mRNA 技术为疫苗学带来了新的前景，目前正在开发和测试针对各种传染病的多种候选疫苗。在这项研究中，我们封装了一种通用的流感 mRNA 疫苗。该疫苗编码流感血凝素 (HA)、核蛋白 (NP) 和基质蛋白 2 (3M2e) 的三个串联重复序列。两次接种疫苗的小鼠在体内表现出强烈的体液和细胞介导的免疫反应。值得注意的是，这些免疫反应导致受挑战小鼠肺部的病毒载量显著降低，并且还赋予了对未来野生型 H1N1、H3N2 或 H5N1 流感病毒挑战的保护。我们的研究结果表明，这种针对流感病毒的 mRNA 通用疫苗策略可能有助于减轻未来流感大流行的影响。
