Mann D R, Smith M M, Gould K G, Collins D C
Fertil Steril. 1985 Jan;43(1):115-21. doi: 10.1016/s0015-0282(16)48328-7.
Three adult male rhesus monkeys were treated for 20 weeks with a gonadotropin-releasing hormone (GnRH) agonist (Ag; Wy-40972; Wyeth Laboratories, Philadelphia, PA) using osmotic minipumps. Ag administration resulted in a transient increase and then a precipitous decrease in the concentration of luteinizing hormone (LH) and testosterone (T). After 5 weeks, serum levels of LH were undetectable (less than 0.2 microgram/ml), while serum T was always detectable, but continued to fall throughout the period of Ag administration. The serum LH and T response to 50 micrograms of GnRH was abolished by 4 weeks of Ag treatment, and this effect persisted through the treatment period. Testicular histology at 20 weeks of Ag treatment exhibited diffuse atrophy of seminiferous tubules, suppressed germinal cell division, and the absence of spermatids or spermatozoa. There was no evidence of testicular necrosis or calcification of the seminiferous tubules. Following the termination of Ag infusion, serum LH and T concentrations rebounded to levels that exceeded pretreatment values for a 5-week period before falling back to baseline levels. A complete restoration of spermatogenesis and testicular volume occurred by 12 weeks after treatment. These data suggest that continuous Ag administration is an effective method of reversibly disrupting spermatogenesis in the male rhesus monkey.
三只成年雄性恒河猴使用渗透微型泵接受促性腺激素释放激素（GnRH）激动剂（Ag；Wy-40972；惠氏实验室，宾夕法尼亚州费城）治疗20周。给予Ag导致促黄体生成素（LH）和睾酮（T）浓度短暂升高，然后急剧下降。5周后，LH血清水平检测不到（低于0.2微克/毫升），而血清T始终可检测到，但在给予Ag期间持续下降。Ag治疗4周后，对50微克GnRH的血清LH和T反应消失，且这种效应在整个治疗期间持续存在。Ag治疗20周时的睾丸组织学显示生精小管弥漫性萎缩、生殖细胞分裂受抑制，且无精子细胞或精子。没有睾丸坏死或生精小管钙化的证据。停止输注Ag后，血清LH和T浓度在反弹至超过治疗前水平5周后回落至基线水平。治疗后12周时精子发生和睾丸体积完全恢复。这些数据表明，持续给予Ag是可逆性破坏雄性恒河猴精子发生的有效方法。
