Biotechnology (BIONORTE), São Luís, Brazil.
Center for Advanced Morphophiological Studies (NEMO), Centre of Agrarian Sciences, State University of the Tocantina Region of Maranhão (UEMASUL), Imperatriz, Brazil.
Open Vet J. 2024 Apr;14(4):962-972. doi: 10.5455/OVJ.2024.v14.i4.3. Epub 2024 Apr 30.
The developmental biology of is described, based on the phenotype. This species is important for the flora because they are excellent seed disseminators. In addition, basic embryological information is not yet fully clarified, and this research provides unprecedented information on the chelonian embryology of the Amazonian fauna.
The present study aims to identify the embryology of in captivity during different periods.
Females were monitored throughout the reproductive cycle, by video monitoring, to identify nests and the presence of newly laid eggs. At regular weekly intervals, embryo samples were collected fixed in a 4% paraformol solution and preserved in 70% alcohol. For the embryonic characterization, we used a stereomicroscope and the scanning electron microscopy method.
We describe 15 embryonic stages for a 15-week (105-day) incubation process. Only at 42 days (6th week) was the morphological characterization of a chelonian observed and at the 12th week (Stage XII), the phenotypic characterization of the species .
In view of the evidence, we found that these phases are similar to the other turtles, with structural variations in the appearance and disappearance of structures due to the specific characteristics of the species.
基于表型描述了 的发育生物学。该物种对植物区系很重要，因为它们是优秀的种子传播者。此外，基本的胚胎学信息尚未完全阐明，本研究为亚马逊动物区系的龟鳖类胚胎学提供了前所未有的信息。
本研究旨在确定圈养 不同时期的胚胎学特征。
通过视频监测，对雌性进行整个生殖周期的监测，以识别巢穴和新产下的蛋的存在。每隔一周定期采集胚胎样本，固定在 4%多聚甲醛溶液中，并保存在 70%的酒精中。为了进行胚胎特征描述，我们使用立体显微镜和扫描电子显微镜方法。
我们描述了 15 个胚胎阶段，孵化过程为 15 周（105 天）。只有在第 42 天（第 6 周）才观察到龟鳖类的形态特征，而在第 12 周（第 XII 阶段）才观察到 的表型特征。
鉴于这些证据，我们发现这些阶段与其他海龟相似，由于物种的特定特征，结构在外观和消失方面存在变化。
