Department of Reproduction, Fertility and Artificial Insemination, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobodjenja 18, Belgrade, 11000, Serbia.
Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobodjenja 18, Belgrade, Serbia.
Vet Res Commun. 2024 Aug;48(4):2429-2440. doi: 10.1007/s11259-024-10426-9. Epub 2024 May 29.
The aim of the study was to investigate the effect of the immunostimulant Mycobacterium Cell Wall Fraction (MCWF) on the treatment of S. aureus SCM by intravenous application. The study included 45 HF dairy cows in 2nd and 3rd month after parturition divided into three groups (n = 15 per group): the MC + group - cows with S. aureus SCM treated with MCWF; the MC- group - cows with S. aureus SCM, with no treatment; and the C group - the control group of healthy cow with no treatment. Samples were collected 0th (I sample), 7th (II), and 14th day (III) from the day of SCM diagnosis and on day 21st (IV). A greater influx of leukocytes was confirmed into milk after 7 days after MCWF treatment in MC + group, which was followed by increase of WBC and LYM in blood. These results support the hypothesis of effective action of MCWF, and in quarters with lower-grade infection, bacteriological cure was achieved. The MC- group had a statistically higher concentration of TBARS and CAT activity in milk, while MC + group had lower blood serum LDH activity, which indicates a positive effect of the MCWF application and a lower exposure of the tissue to lipide peroxidation and inflammation caused by S. aureus. The application of MCWF would give new possibilities in the prevention and therapy of mammary gland diseases without fear of the presence of residues and the emergence of bacterial resistance. In future studies, the effects of local and systemic application of MCWF in the treatment of S. aureus SCM should be compared.
本研究旨在通过静脉应用免疫刺激剂分枝杆菌细胞壁片段（MCWF），探讨其对金黄色葡萄球菌乳房炎（S. aureus SCM）治疗的影响。该研究纳入了产后第 2 至 3 个月的 45 头荷斯坦奶牛，分为三组（每组 15 头）：MC+组-用 MCWF 治疗的金黄色葡萄球菌 SCM 奶牛；MC-组-金黄色葡萄球菌 SCM 奶牛，未治疗；C 组-未治疗的健康奶牛对照组。在 SCM 确诊当天及第 7 天（II）和第 14 天（III）及第 21 天（IV）收集样本。MC+组在 MCWF 治疗后第 7 天确认白细胞更多地涌入乳汁，随后血液中的 WBC 和 LYM 增加。这些结果支持 MCWF 有效作用的假设，并且在感染程度较低的乳区实现了细菌学治愈。MC-组乳汁中 TBARS 和 CAT 活性的浓度统计学上更高，而 MC+组血液中 LDH 活性较低，这表明 MCWF 应用具有积极作用，金黄色葡萄球菌引起的组织脂质过氧化和炎症暴露较低。应用 MCWF 将为预防和治疗乳腺炎提供新的可能性，而无需担心残留和细菌耐药性的出现。在未来的研究中，应比较 MCWF 局部和全身应用在金黄色葡萄球菌 SCM 治疗中的效果。
