耐酸碱重组漆酶与黄曲霉毒素 B 和玉米赤霉烯酮的降解潜力。
Thermo-Alkali-Tolerant Recombinant Laccase from and Its Degradation Potential against Zearalenone and Aflatoxin B.
机构信息
College of Food Science and Engineering, Henan Key Laboratory of Cereal and Oil Food Safety and Nutrition, Henan University of Technology, Zhengzhou, Henan 450001, China.
Department of Food Science and Technology, College of Agricultural Sciences and Technology, Mbeya University of Science and Technology, P.O. Box 131, Mbeya 53119, Tanzania.
出版信息
J Agric Food Chem. 2024 Jun 12;72(23):13371-13381. doi: 10.1021/acs.jafc.4c01304. Epub 2024 May 29.
The enzymatic biodegradation of mycotoxins in food and feed has attracted the most interest in recent years. In this paper, the laccase gene from was cloned and expressed in BL 21(D3). The sequence analysis indicated that the gene consisted of 1533 bp. The purified laccase was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis -12% with an estimated molecular weight of 56.7 kDa. The enzyme is thermo-alkali-tolerant, displaying the optimal degradation of zearalenone (ZEN) and aflatoxin B (AFB) at pH 8 and 9, with incubation temperatures of 55 and 50 °C, respectively, within 24 h. The degradation potentials of the 50 μg of the enzyme against ZEN (5.0 μg/mL) and AFB (2.5 μg/mL) were 99.60 and 96.73%, respectively, within 24 h. To the best of our knowledge, this is the first study revealing the recombinant production of laccase from , its biochemical properties, and potential use in ZEN and AFB degradation in vitro and in vivo.
近年来，食品和饲料中真菌毒素的酶法生物降解引起了人们的极大兴趣。本文从 中克隆并表达了漆酶基因 BL 21(D3)。序列分析表明，该基因由 1533bp 组成。经 12%十二烷基硫酸钠-聚丙烯酰胺凝胶电泳纯化后，酶的分子量约为 56.7 kDa。该酶具有耐热耐碱的特性，在 pH8 和 9 下，55°C 和 50°C 孵育 24 小时，对玉米赤霉烯酮（ZEN）和黄曲霉毒素 B（AFB）的最佳降解分别为 99.60%和 96.73%。在 24 小时内，50μg 该酶对 5.0μg/mL 的 ZEN 和 2.5μg/mL 的 AFB 的降解率分别为 99.60%和 96.73%。据我们所知，这是首次从 中成功表达漆酶，研究其生化特性，并探讨其在体外和体内降解 ZEN 和 AFB 的潜在用途。
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